| Literature DB >> 28349626 |
N I Gorshkov1, А Е Miroslavov2,3, I Е Аlekseev2, А А Lumpov2, А Y Мurko1, I I Gavrilova1, N N Saprykina1, М А Bezrukova1, А I Kipper1, V D Krasikov1, D N Suglobov2, M Y Tyupina2, Е F Panarin4.
Abstract
The procedure of the directed synthesis of N-vinylpyrrolidone-N-vinylformamide (VP-VFA) copolymers with grafted iminodiacetate (IDA) chelating units is presented. The methods for labelling resulting conjugates with indium-113m were developed. The metal-copolymer conjugates were characterized by different physicochemical methods, including IR and NMR, viscometry, light scattering, and exclusion high-performance liquid chromatography. Parameters of radiochemical synthesis of the conjugates labelled with indium-113m were optimized. It was shown that the VP-VFA-IDA copolymer firmly binds indium-113m both in the acid and alkaline solutions, with pH of the reaction mixture having almost no effect on the complexation. VP-VFA-IDA-In conjugates were found to be unstable in histidine challenge reaction.Entities:
Keywords: N-vinylpyrrolidone-N-vinylformamide copolymer; bioconjugates; indium-113m; labelling; radiochemical synthesis; site specific radiopharmaceuticals
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Year: 2017 PMID： 28349626 DOI： 10.1002/jlcr.3503
Source DB: PubMed Journal: J Labelled Comp Radiopharm ISSN： 0362-4803 Impact factor: 1.921