Literature DB >> 28322799

Novel method for measurement of heparin anticoagulant activity using SPR.

Jing Zhao1, Xinyue Liu2, Anju Malhotra3, Quanhong Li4, Fuming Zhang5, Robert J Linhardt6.   

Abstract

A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.
Copyright © 2017 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Anticoagulant activity; Antithrombin III; Heparin; SPR

Mesh：

Substances：

Year:  2017        PMID： 28322799      PMCID： PMC5553204          DOI： 10.1016/j.ab.2017.03.013

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

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4.  The purification and mechanism of action of human antithrombin-heparin cofactor.

Authors:  R D Rosenberg; P S Damus
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6.  Investigation of the Differences in Antithrombin to Heparin Binding among Antithrombin Budapest 3, Basel, and Padua Mutations by Biochemical and In Silico Methods.

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