| Literature DB >> 28321791 |
Maria Gabriela Xavier Oliveira1, Leandro Nogueira Pressinotti2, Giovane Spínola Carvalho2, Mirela Caroline Vilela Oliveira1, Luisa Zanolli Moreno1, Carlos Emilio Cabrera Matajira1, Alessandro Spínola Bergamo2, Victor Manuel Aleixo3, Alexandre Caixeta Veiga2, Elvis de Souza Corsino3, Ana Paula Guarnieri Christ4, Maria Inês Zanolli Sato4, Andrea Micke Moreno1, Terezinha Knöbl5.
Abstract
The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.Entities:
Keywords: Alligators; Campylobacteriaceae; Pathogens; Reptile
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Year: 2017 PMID： 28321791 DOI： 10.1007/s11250-017-1262-3
Source DB: PubMed Journal: Trop Anim Health Prod ISSN： 0049-4747 Impact factor: 1.559