| Literature DB >> 2831880 |
Y Nagao1, T Nakada, M Imoto, T Shimamoto, S Sakai, M Tsuda, T Tsuchiya.
Abstract
Alpha-Galactosidase, the product of the melA gene, was purified from a strain of Escherichia coli harboring a plasmid carrying melA, which over-produced the alpha-galactosidase. An apparent molecular weight was determined to be 50 kDa. The amino acid composition of this enzyme was determined. The result indicates that this enzyme is a hydrophilic and acidic protein. We have subjected the purified enzyme to 20 cycles of N-terminal sequence analysis. This verified the translation start site of the melA gene and the predicted N-terminal sequence.Entities:
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Year: 1988 PMID： 2831880 DOI： 10.1016/0006-291x(88)90584-0
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575