| Literature DB >> 28318608 |
Roger Dumke1, Alvaro J Benitez2, Victoria Chalker3, Karolina Gullsby4, Birgit Henrich5, Carlos Hidalgo-Grass6, Theo Hoogenboezem7, Darja Kese8, Katherine Loens9, Jolanda Maaskant10, Ayelet Michael-Gayego11, Allon E Moses11, Ran Nir-Paz11, Suzan D Pas10, Sabine Pereyre12, Randi F Petersen13, Meike Rosenblatt5, Annemarie M C van Rossum14, Søren A Uldum13, Wendy W J Unger7, Dominique Ursi9, Jonas M Winchell2, Cecile Bebear12.
Abstract
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.Entities:
Keywords: Detection; Multi-center evaluation; Mycoplasma pneumoniae; Real-time PCR
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Year: 2017 PMID： 28318608 DOI： 10.1016/j.diagmicrobio.2017.03.004
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803