Literature DB >> 28317990

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase.

Li-Juan Wang1, Zi-Yue Wang1, Qianyi Zhang2, Bo Tang1, Chun-Yang Zhang1.   

Abstract

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 × 10-7 U μL-1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.

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Year:  2017        PMID： 28317990     DOI： 10.1039/c7cc00946a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

Review 1.  Fluorescent Probes of DNA Repair.

Authors:  David L Wilson; Eric T Kool
Journal:  ACS Chem Biol       Date:  2017-11-30       Impact factor: 5.100

2.  Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites.

Authors:  Zi-Yue Wang; Li-Juan Wang; Qianyi Zhang; Bo Tang; Chun-Yang Zhang
Journal:  Chem Sci       Date:  2017-12-13       Impact factor: 9.825

Review 3.  Inhibitors of DNA Glycosylases as Prospective Drugs.

Authors:  Grigory V Mechetin; Anton V Endutkin; Evgeniia A Diatlova; Dmitry O Zharkov
Journal:  Int J Mol Sci       Date:  2020-04-28       Impact factor: 5.923

4.  Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells.

Authors:  Li-Juan Wang; Ming-Li Luo; Xiao-Yun Yang; Xiao-Fang Li; Yanxia Wu; Chun-Yang Zhang
Journal:  Theranostics       Date:  2019-06-09       Impact factor: 11.556

5.  Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase.

Authors:  Li-Juan Wang; Xiao Han; Chen-Chen Li; Chun-Yang Zhang
Journal:  Chem Sci       Date:  2018-06-18       Impact factor: 9.825

6.  Construction of a self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background.

Authors:  Li-Juan Wang; Ying-Ying Lu; Chun-Yang Zhang
Journal:  Chem Sci       Date:  2019-11-26       Impact factor: 9.825
  6 in total

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