Literature DB >> 28305833

A quantitative method for separation of livingHydra cells.

Martin J Greber1, Charles N David1, Thomas W Holstein1.   

Abstract

We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.

Keywords:  Cell separation; Cell type specific gene expression; Counterflow centrifugation elutriation; Hydra

Year:  1992        PMID： 28305833     DOI： 10.1007/BF00592110

Source DB:  PubMed          Journal:  Rouxs Arch Dev Biol        ISSN： 0930-035X


  14 in total

1.  Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells.

Authors:  T W Holstein; C N David
Journal:  Dev Biol       Date:  1990-12       Impact factor: 3.582

2.  Cell cycle length, cell size, and proliferation rate in hydra stem cells.

Authors:  T W Holstein; C N David
Journal:  Dev Biol       Date:  1990-12       Impact factor: 3.582

3.  A quantitative method for maceration of hydra tissue.

Authors:  Charles N David
Journal:  Wilhelm Roux Arch Entwickl Mech Org       Date:  1973-12

4.  Quantitative analysis of cell types during growth and morphogenesis in Hydra.

Authors:  H Bode; S Berking; C N David; A Gierer; H Schaller; E Trenkner
Journal:  Wilhelm Roux Arch Entwickl Mech Org       Date:  1973-12

5.  Neuron differentiation in hydra involves dividing intermediates.

Authors:  H R Bode; L W Gee; M A Chow
Journal:  Dev Biol       Date:  1990-06       Impact factor: 3.582

6.  Regeneration of hydra from reaggregated cells.

Authors:  A Gierer; S Berking; H Bode; C N David; K Flick; G Hansmann; H Schaller; E Trenkner
Journal:  Nat New Biol       Date:  1972-09-27

7.  Distribution of the head-activating substance in hydra and its localization in membranous particles in nerve cells.

Authors:  H Schaller; A Gierer
Journal:  J Embryol Exp Morphol       Date:  1973-02

8.  The morphogenesis of nematocytes in Hydra and Forskålia: an ultrastructural study.

Authors:  T Holstein
Journal:  J Ultrastruct Res       Date:  1981-06

9.  suc1 is an essential gene involved in both the cell cycle and growth in fission yeast.

Authors:  J Hayles; S Aves; P Nurse
Journal:  EMBO J       Date:  1986-12-01       Impact factor: 11.598

10.  Mini-collagens in hydra nematocytes.

Authors:  E M Kurz; T W Holstein; B M Petri; J Engel; C N David
Journal:  J Cell Biol       Date:  1991-11       Impact factor: 10.539
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  3 in total

1.  Stem cell differentiation trajectories in Hydra resolved at single-cell resolution.

Authors:  Stefan Siebert; Jeffrey A Farrell; Jack F Cazet; Yashodara Abeykoon; Abby S Primack; Christine E Schnitzler; Celina E Juliano
Journal:  Science       Date:  2019-07-26       Impact factor: 47.728

2.  Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry.

Authors:  Wanda Buzgariu; Jean-Pierre Aubry-Lachainaye; Brigitte Galliot
Journal:  Methods Mol Biol       Date:  2022

3.  Temperature and insulin signaling regulate body size in Hydra by the Wnt and TGF-beta pathways.

Authors:  Benedikt M Mortzfeld; Jan Taubenheim; Alexander V Klimovich; Sebastian Fraune; Philip Rosenstiel; Thomas C G Bosch
Journal:  Nat Commun       Date:  2019-07-22       Impact factor: 14.919
  3 in total

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