Mariana de Castro Nicodemo1, Lia Renó das Neves2, Josafá Carvalho Aguiar3, Flaviane de Souza Brito4, Isabelle Ferreira5, Luciana Barros Sant'Anna6, Leandro José Raniero5, Rodrigo Álvaro Lopes Martins7, Paulo Roxo Barja8, Emilia Angela Lo Schiavo Arisawa9. 1. Postgraduate Program in Biomedical Engineering, Universidade do Vale do Paraíba, São José dos Campos, SP, Brazil. 2. Postgraduate Program in Biomedical Engineering, UNIVAP, São José dos Campos, SP, Brazil. 3. Postgraduate Program in Physics, UNIVAP, São José dos Campos, SP, Brazil. 4. Postgraduate Program in Biological Sciences, UNIVAP, São José dos Campos, SP, Brazil. 5. Nanosensors Laboratory, UNIVAP, São José dos Campos, SP, Brazil. 6. Immunology Laboratory, UNIVAP, São José dos Campos, SP, Brazil. 7. Postgraduate Program in Biomedical Engineering, Technology Research Center, Universidade Mogi das Cruzes, Brazil. 8. Photoacoustic Applied to Biological Systems Laboratory, UNIVAP, São José dos Campos, SP, Brazil. 9. Biostimulation and Tissue Repair Laboratory, UNIVAP, São José dos Campos, SP, Brazil.
Abstract
PURPOSE: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. METHODS: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. RESULTS: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. CONCLUSION: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.
PURPOSE: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. METHODS: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. RESULTS: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. CONCLUSION: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.
Authors: Wei Lee Lim; Ling Ling Liau; Min Hwei Ng; Shiplu Roy Chowdhury; Jia Xian Law Journal: Tissue Eng Regen Med Date: 2019-06-26 Impact factor: 4.169
Authors: J Freeland Ackley; Michael Kolosky; Danielle Gurin; Robert Hampton; Richard Masin; David Krahe Journal: Medicine (Baltimore) Date: 2019-07 Impact factor: 1.817