Literature DB >> 2829254

A low copy number cosmid.

X M Jiang1, H N Brahmbhatt, N B Quigley, P R Reeves.   

Abstract

A low copy number cosmid was constructed by subcloning the pair of cos sites and the kanamycin resistance gene of pcos2EMBL into pGB2. The resulting cosmid, pPR691, has the pSC101 replicon and specifies resistance to kanamycin, spectinomycin, and streptomycin. pPR691 also carries restriction sites suitable for cloning partial Sau3A digests using the strategy of Bates and Swift (P. F. Bates and R. A. Swift, 1983, Gene 26, 137-146). A library of Salmonella typhimurium chromosomal DNA was made using this cosmid and the rfb gene cluster (map position 42) was isolated from this library.

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Year:  1987        PMID： 2829254     DOI： 10.1016/0147-619x(87)90045-x

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  7 in total

1.  Functional analysis of genes in the rfb locus of Leptospira borgpetersenii serovar Hardjo subtype Hardjobovis.

Authors:  D M Bulach; T Kalambaheti; A de la Peña-Moctezuma; B Adler
Journal:  Infect Immun       Date:  2000-07       Impact factor: 3.441

2.  New vector tools with a hygromycin resistance marker for use with opportunistic pathogens.

Authors:  Eric J Kalivoda; Joseph Horzempa; Nicholas A Stella; Aroba Sadaf; Regis P Kowalski; Gerard J Nau; Robert M Q Shanks
Journal:  Mol Biotechnol       Date:  2011-05       Impact factor: 2.695

3.  Use of Salmonella phage P22 for transduction in Escherichia coli.

Authors:  B L Neal; P K Brown; P R Reeves
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

4.  Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains.

Authors:  S H Xiang; M Hobbs; P R Reeves
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

5.  Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library.

Authors:  S Purton; J D Rochaix
Journal:  Plant Mol Biol       Date:  1994-02       Impact factor: 4.076

6.  The capsule polysaccharide synthesis locus of streptococcus pneumoniae serotype 14: Identification of the glycosyl transferase gene cps14E.

Authors:  M A Kolkman; D A Morrison; B A Van Der Zeijst; P J Nuijten
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

7.  New yeast recombineering tools for bacteria.

Authors:  Robert M Q Shanks; Daniel E Kadouri; Daniel P MacEachran; George A O'Toole
Journal:  Plasmid       Date:  2009-05-27       Impact factor: 3.466
  7 in total

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