Niki Christou1,2,3, Aurélie Perraud4,5,6, Sabrina Blondy4,5, Marie-Odile Jauberteau4,5, Serge Battu4,5, Muriel Mathonnet4,5,6. 1. Laboratoire EA 3842, Homéostasie cellulaire et Pathologies, Faculté de Médecine et de Pharmacie, Université de Limoges, 2 rue du Dr Marcland, 87025, Limoges Cedex, France. christou.niki19@gmail.com. 2. Institut Fédératif de Recherche 145 GEIST « Génomique, Environnement, Immunité, Santé et Thérapeutiques », Université de Limoges, 2 rue du Dr Marcland, 87025, Limoges Cedex, France. christou.niki19@gmail.com. 3. Service de chirurgie digestive générale et endocrinienne, CHRU de Limoges, 2 rue Martin Luther King, 87042, Limoges Cedex, France. christou.niki19@gmail.com. 4. Laboratoire EA 3842, Homéostasie cellulaire et Pathologies, Faculté de Médecine et de Pharmacie, Université de Limoges, 2 rue du Dr Marcland, 87025, Limoges Cedex, France. 5. Institut Fédératif de Recherche 145 GEIST « Génomique, Environnement, Immunité, Santé et Thérapeutiques », Université de Limoges, 2 rue du Dr Marcland, 87025, Limoges Cedex, France. 6. Service de chirurgie digestive générale et endocrinienne, CHRU de Limoges, 2 rue Martin Luther King, 87042, Limoges Cedex, France.
Abstract
PURPOSE: Multiple studies have attempted to demonstrate the interest of the cell adhesion marker, E cadherin, as a diagnostic and prognosis marker in colorectal cancer (CRC). However, it was considered non specific. MATERIALS AND METHODS: Studies were carried out with CRC cell lines and patients' cohort operated for CRC. The expression of E cadherin was studied after 5 fluorouracil (5FU) treatment and correlated to CRC relapse, chemo-resistance and survival. RESULTS: In CRC cell lines derived from high tumor stages, extracellular domain of E cadherin expression decreased after 5FU treatment whereas it increased in supernatants. Interestingly, only specific cleaved forms at 55 kDa of E cadherin were detected in supernatants. In CRC surgical patients, more importantly concerning extracellular E cadherin domain, a decreased expression was observed in tissues in function of CRC stages whereas an increased expression was found in sera. Moreover, there is an increasing trend of survival with weak serum E cadherin secretion, reinforcing the implication of this protein in CRC evolution. DISCUSSION: The extracellular domain can be defined as a 5FU resistance marker and allow CRC monitoring.
PURPOSE: Multiple studies have attempted to demonstrate the interest of the cell adhesion marker, E cadherin, as a diagnostic and prognosis marker in colorectal cancer (CRC). However, it was considered non specific. MATERIALS AND METHODS: Studies were carried out with CRC cell lines and patients' cohort operated for CRC. The expression of E cadherin was studied after 5 fluorouracil (5FU) treatment and correlated to CRC relapse, chemo-resistance and survival. RESULTS: In CRC cell lines derived from high tumor stages, extracellular domain of E cadherin expression decreased after 5FU treatment whereas it increased in supernatants. Interestingly, only specific cleaved forms at 55 kDa of E cadherin were detected in supernatants. In CRC surgical patients, more importantly concerning extracellular E cadherin domain, a decreased expression was observed in tissues in function of CRC stages whereas an increased expression was found in sera. Moreover, there is an increasing trend of survival with weak serum E cadherin secretion, reinforcing the implication of this protein in CRC evolution. DISCUSSION: The extracellular domain can be defined as a 5FU resistance marker and allow CRC monitoring.
Entities:
Keywords:
Colorectal cancer; E cadherin; Extracellular domain; Serum
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