| Literature DB >> 28287531 |
Enrica Bertin1, Martina Piccoli1, Chiara Franzin1, Andras Nagy2, Maria Mileikovsky2, Paolo De Coppi3, Michela Pozzobon4.
Abstract
Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.Entities:
Mesh:
Year: 2017 PMID: 28287531 PMCID: PMC5407613 DOI: 10.3791/54598
Source DB: PubMed Journal: J Vis Exp ISSN: 1940-087X Impact factor: 1.355