| Literature DB >> 28280154 |
Leslie A Mitchell1,2, Ann Wang3,4, Giovanni Stracquadanio3,5,6, Zheng Kuang1,2, Xuya Wang1,2, Kun Yang3,5, Sarah Richardson3,5, J Andrew Martin1,2, Yu Zhao1,2, Roy Walker7, Yisha Luo7, Hongjiu Dai8, Kang Dong8, Zuojian Tang1,2, Yanling Yang9, Yizhi Cai7, Adriana Heguy10, Beatrix Ueberheide1,9, David Fenyö1,2, Junbiao Dai4, Joel S Bader3,4, Jef D Boeke11,2.
Abstract
We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the HIS2 transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain "omics" analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome.Entities:
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Year: 2017 PMID： 28280154 DOI： 10.1126/science.aaf4831
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728