Yavuz Tokgöz1, Ishak Abdurrahman Işık2, Soheil Akbari3, Tuncay Kume4, Oya Sayın5, Esra Erdal3, Nur Arslan6. 1. Department of Pediatric Gastroenterology, Hepatology and Nutrition, Dokuz Eylul University Faculty of Medicine, 35330, Inciraltı-Izmir, Turkey. drytokgoz@yahoo.com. 2. Department of Pediatric Gastroenterology, Hepatology and Nutrition, Dokuz Eylul University Faculty of Medicine, 35330, Inciraltı-Izmir, Turkey. 3. Department of Medical Biology, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. 4. Department of Biochemistry, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. 5. Research Laboratory, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. 6. Department of Pediatric Metabolism and Nutrition, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey.
Abstract
BACKGROUND: The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. METHODS: A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T] were studied by Real Time-PCR. Serum leptin, adiponectin, resistin and ghrelin levels were studied by ELISA method in both groups and their link with perilipin polymorphisms were analyzed. RESULTS: Serum leptin level was found significantly high in obese adolescents. Other adipokine levels were similar in both groups. The incidence of PLIN 1, PLIN 4, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T minor and major alleles was similar in both groups. PLIN 6 T/T allele was determined significantly high in obese adolescents compared to that of control group. No correlation was detected between perilipin polymorphism and serum levels of adipokines. CONCLUSION: The PLIN 6 polymorphism of the perilipin gene may influence the risk of the obesity during adolescence. TRIAL REGISTRATION: Retrospectively registered.
BACKGROUND: The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. METHODS: A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5'UTR-1234 C > G and PLIN10171 A/T] were studied by Real Time-PCR. Serum leptin, adiponectin, resistin and ghrelin levels were studied by ELISA method in both groups and their link with perilipin polymorphisms were analyzed. RESULTS: Serum leptin level was found significantly high in obese adolescents. Other adipokine levels were similar in both groups. The incidence of PLIN 1, PLIN 4, PLIN 5'UTR-1234 C > G and PLIN10171 A/T minor and major alleles was similar in both groups. PLIN 6 T/T allele was determined significantly high in obese adolescents compared to that of control group. No correlation was detected between perilipin polymorphism and serum levels of adipokines. CONCLUSION: The PLIN 6 polymorphism of the perilipin gene may influence the risk of the obesity during adolescence. TRIAL REGISTRATION: Retrospectively registered.
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