Julieta Trinks1,2, Nao Nishida3,4, María Laura Hulaniuk1, Mariela Caputo2,5, Takayo Tsuchiura3, Sebastián Marciano6, Leila Haddad6, Jorgelina Blejer7, Sonia Bartoli8, Beatriz Ameigeiras9, Silvia E Frías9, Cecilia Vistarini9, Fabiana Heinrich10, Carlos Remondegui11, Susana Ceballos11, Gustavo Echenique12, Miguel Charre Samman13, Claudia D'Amico14, Amalia Rojas14, Alfredo Martínez15, Ezequiel Ridruejo15, Roberto J Fernández7, Leandro Burgos Pratx16, Horacio Salamone16, Félix Nuñez16, Omar Galdame6, Adrián Gadano6, Daniel Corach2,5, Masaya Sugiyama3, Diego Flichman2,17, Katsushi Tokunaga4, Masashi Mizokami3. 1. Basic Sciences and Experimental Medicine Institute (ICBME), University Institute of the Italian Hospital of Buenos Aires, Buenos Aires, Argentina. 2. Scientific and Technological Research Council (CONICET), Buenos Aires, Argentina. 3. Department of Hepatic Disease, Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Chiba, Japan. 4. Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. 5. Genetic Fingerprints Unit, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. 6. Hepatology Unit, Italian Hospital of Buenos Aires, Buenos Aires, Argentina. 7. "Hemocentro Buenos Aires" Foundation, Buenos Aires, Argentina. 8. Hemotherapy Unit, "Pablo Soria" Hospital, San Salvador de Jujuy, Argentina. 9. Hepatology Unit, "José María Ramos Mejía" General Hospital, Buenos Aires, Argentina. 10. "San Antonio" Hospital, Gualeguay, Argentina. 11. Infectology and Tropical Medicine Unit, "San Roque" Hospital, San Salvador de Jujuy, Argentina. 12. "Nuestra Señora del Rosario" Clinic, San Salvador de Jujuy, Argentina. 13. Central Public Health Laboratory, San Salvador de Jujuy, Argentina. 14. Ambulatory Medical Specialities Center (CEMA), Mar del Plata, Argentina. 15. Center for Medical Education and Clinical Research "Norberto Quirno" (CEMIC), Buenos Aires, Argentina. 16. Transfusional Medicine Unit, Italian Hospital of Buenos Aires, Buenos Aires, Argentina. 17. Virology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
Abstract
BACKGROUND & AIMS: HBV infection exhibits geographical variation in its distribution in South America. While HBV rates are low in central Argentina, the north-western region exhibits intermediate HBV rates. Unfortunately, the reasons that could explain this difference are still unknown. METHODS: A total of 1440 Argentines were recruited and grouped into HBV patients, HBV-resolved individuals and healthy controls. Genetic ancestry was assessed by analysis of biparental lineages and ancestry autosomal typing. SNPs of HLA-DPA1 (rs3077), HLA-DPB1 (rs9277542), HLA-DQB1 (rs2856718) and HLA-DQB2 (rs7453920) were determined, and HBV genotyping was performed by phylogenetic analysis in HBV patients. RESULTS: Native American ancestry prevailed in the north-western region when compared with central Argentina (P<.0001). However, no differences were observed among the three groups of each region. The distribution of HBV genotypes revealed significant differences (P<.0001). Three SNPs (rs3077, rs9277542 and rs7453920) showed a significant association with protection against chronic HBV and viral clearance in both regions. The remaining SNP showed a significant association with susceptibility to chronic HBV. The frequency rates of rs3077-T, related to protection against chronic HBV and viral clearance, were lower in north-western Argentina when compared with central Argentina. The same uneven frequency rates were observed for SNP rs9277542. CONCLUSIONS: This is the first study addressing the associations between the HLA-DP and HLA-DQ loci and the protection against chronic HBV and viral clearance in a multiethnic South American population. The uneven distribution of HLA-DP and HLA-DQ supports the HBV epidemiological differences observed in these two regions of Argentina with dissimilar ancestry genetic background.
BACKGROUND & AIMS:HBV infection exhibits geographical variation in its distribution in South America. While HBV rates are low in central Argentina, the north-western region exhibits intermediate HBV rates. Unfortunately, the reasons that could explain this difference are still unknown. METHODS: A total of 1440 Argentines were recruited and grouped into HBV patients, HBV-resolved individuals and healthy controls. Genetic ancestry was assessed by analysis of biparental lineages and ancestry autosomal typing. SNPs of HLA-DPA1 (rs3077), HLA-DPB1 (rs9277542), HLA-DQB1 (rs2856718) and HLA-DQB2 (rs7453920) were determined, and HBV genotyping was performed by phylogenetic analysis in HBV patients. RESULTS: Native American ancestry prevailed in the north-western region when compared with central Argentina (P<.0001). However, no differences were observed among the three groups of each region. The distribution of HBV genotypes revealed significant differences (P<.0001). Three SNPs (rs3077, rs9277542 and rs7453920) showed a significant association with protection against chronic HBV and viral clearance in both regions. The remaining SNP showed a significant association with susceptibility to chronic HBV. The frequency rates of rs3077-T, related to protection against chronic HBV and viral clearance, were lower in north-western Argentina when compared with central Argentina. The same uneven frequency rates were observed for SNP rs9277542. CONCLUSIONS: This is the first study addressing the associations between the HLA-DP and HLA-DQ loci and the protection against chronic HBV and viral clearance in a multiethnic South American population. The uneven distribution of HLA-DP and HLA-DQ supports the HBV epidemiological differences observed in these two regions of Argentina with dissimilar ancestry genetic background.
Authors: María B Pisano; Cecilia G Giadans; Diego M Flichman; Viviana E Ré; María V Preciado; Pamela Valva Journal: World J Gastroenterol Date: 2021-07-14 Impact factor: 5.742