Literature DB >> 2826539

Serum albumin treatment to eliminate false-positive results with laboratory rodent specimens tested by rotazyme enzyme-linked immunosorbent assay.

M N Jure1, S S Morse, D M Stark.   

Abstract

A high proportion of positive results was found in fecal specimens from mice and rats tested by Rotazyme II enzyme-linked immunosorbent assay, but the presence of virus could not be confirmed. Positive specimens and nonautoclaved rodent diet contained a substance that apparently attached nonspecifically to the antibody-coated beads used in the test and reacted directly with the substrate. Pretreatment of the beads with 0.1% bovine serum albumin eliminated this nonspecific activity.

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Year:  1987        PMID： 2826539      PMCID： PMC269455          DOI： 10.1128/jcm.25.11.2239-2240.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

2.  Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.

Authors:  M Chernesky; S Castriciano; J Mahony; D DeLong
Journal:  J Clin Microbiol       Date:  1985-09       Impact factor: 5.948

3.  Cultivation and characterization of three strains of murine rotavirus.

Authors:  H B Greenberg; P T Vo; R Jones
Journal:  J Virol       Date:  1986-02       Impact factor: 5.103

4.  Rotavirus-associated diarrhea in a commercial rabbitry.

Authors:  T R Schoeb; D B Casebolt; V E Walker; L N Potgieter; M E Thouless; R F DiGiacomo
Journal:  Lab Anim Sci       Date:  1986-04

Review 5.  Rotaviruses.

Authors:  M S McNulty
Journal:  J Gen Virol       Date:  1978-07       Impact factor: 3.891

6.  Synergistic rotavirus and Escherichia coli diarrheal infection of mice.

Authors:  P M Newsome; K A Coney
Journal:  Infect Immun       Date:  1985-02       Impact factor: 3.441

7.  Unreliability of Rotazyme ELISA test in neonates.

Authors:  P J Krause; J S Hyams; P J Middleton; V C Herson; J Flores
Journal:  J Pediatr       Date:  1983-08       Impact factor: 4.406

Review 8.  Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects.

Authors:  R H Yolken
Journal:  Rev Infect Dis       Date:  1982 Jan-Feb

9.  Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.

Authors:  A S Rubenstein; M F Miller
Journal:  J Clin Microbiol       Date:  1982-05       Impact factor: 5.948

10.  The rotaviruses.

Authors:  T H Flewett; G N Woode
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574
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