OBJECTIVE: BR55, a vascular endothelial growth factor receptor 2 (VEGFR2)-specific ultrasound molecular contrast agent (MCA), has shown promising results in multiple preclinical models regarding cancer imaging. In this first-in-human, phase 0, exploratory study, we investigated the feasibility and safety of the MCA for the detection of prostate cancer (PCa) in men using clinical standard technology. MATERIALS AND METHODS: Imaging with the MCA was performed in 24 patients with biopsy-proven PCa scheduled for radical prostatectomy using a clinical ultrasound scanner at low acoustic power. Safety monitoring was done by physical examination, blood pressure and heart rate measurements, electrocardiogram, and blood sampling. As first-in-human study, MCA dosing and imaging protocol were necessarily fine-tuned along the enrollment to improve visualization. Imaging data were correlated with radical prostatectomy histopathology to analyze the detection rate of ultrasound molecular imaging with the MCA. RESULTS: Imaging with MCA doses of 0.03 and 0.05 mL/kg was adequate to obtain contrast enhancement images up to 30 minutes after administration. No serious adverse events or clinically meaningful changes in safety monitoring data were identified during or after administration. BR55 dosing and imaging were fine-tuned in the first 12 patients leading to 12 subsequent patients with an improved MCA dosing and imaging protocol. Twenty-three patients underwent radical prostatectomy. A total of 52 lesions were determined to be malignant by histopathology with 26 (50%) of them seen during BR55 imaging. In the 11 patients that were scanned with the improved protocol and underwent radical prostatectomy, a total of 28 malignant lesions were determined: 19 (68%) were seen during BR55 ultrasound molecular imaging, whereas 9 (32%) were not identified. CONCLUSIONS: Ultrasound molecular imaging with BR55 is feasible with clinical standard technology and demonstrated a good safety profile. Detectable levels of the MCA can be reached in patients with PCa opening the way for further clinical trials.
OBJECTIVE: BR55, a vascular endothelial growth factor receptor 2 (VEGFR2)-specific ultrasound molecular contrast agent (MCA), has shown promising results in multiple preclinical models regarding cancer imaging. In this first-in-human, phase 0, exploratory study, we investigated the feasibility and safety of the MCA for the detection of prostate cancer (PCa) in men using clinical standard technology. MATERIALS AND METHODS: Imaging with the MCA was performed in 24 patients with biopsy-proven PCa scheduled for radical prostatectomy using a clinical ultrasound scanner at low acoustic power. Safety monitoring was done by physical examination, blood pressure and heart rate measurements, electrocardiogram, and blood sampling. As first-in-human study, MCA dosing and imaging protocol were necessarily fine-tuned along the enrollment to improve visualization. Imaging data were correlated with radical prostatectomy histopathology to analyze the detection rate of ultrasound molecular imaging with the MCA. RESULTS: Imaging with MCA doses of 0.03 and 0.05 mL/kg was adequate to obtain contrast enhancement images up to 30 minutes after administration. No serious adverse events or clinically meaningful changes in safety monitoring data were identified during or after administration. BR55 dosing and imaging were fine-tuned in the first 12 patients leading to 12 subsequent patients with an improved MCA dosing and imaging protocol. Twenty-three patients underwent radical prostatectomy. A total of 52 lesions were determined to be malignant by histopathology with 26 (50%) of them seen during BR55 imaging. In the 11 patients that were scanned with the improved protocol and underwent radical prostatectomy, a total of 28 malignant lesions were determined: 19 (68%) were seen during BR55 ultrasound molecular imaging, whereas 9 (32%) were not identified. CONCLUSIONS: Ultrasound molecular imaging with BR55 is feasible with clinical standard technology and demonstrated a good safety profile. Detectable levels of the MCA can be reached in patients with PCa opening the way for further clinical trials.
Authors: Johann Le Floc'h; Aimen Zlitni; Holly A Bilton; Melissa Yin; Arash Farhadi; Nancy R Janzen; Mikhail G Shapiro; John F Valliant; F Stuart Foster Journal: Mol Imaging Biol Date: 2018-04 Impact factor: 3.488
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Authors: Dongwoon Hyun; Lotfi Abou-Elkacem; Rakesh Bam; Leandra L Brickson; Carl D Herickhoff; Jeremy J Dahl Journal: IEEE Trans Med Imaging Date: 2020-04-09 Impact factor: 10.048
Authors: Miao Li; Dawei Jiang; Todd E Barnhart; Tianye Cao; Jonathan W Engle; Weiyu Chen; Weibo Cai Journal: Am J Cancer Res Date: 2019-09-01 Impact factor: 6.166
Authors: Tomasz J Czernuszewicz; Virginie Papadopoulou; Juan D Rojas; Rajalekha M Rajamahendiran; Jonathan Perdomo; James Butler; Max Harlacher; Graeme O'Connell; Dženan Zukić; Stephen R Aylward; Paul A Dayton; Ryan C Gessner Journal: Rev Sci Instrum Date: 2018-07 Impact factor: 1.523
Authors: Juan D Rojas; Virginie Papadopoulou; Tomasz J Czernuszewicz; Rajalekha M Rajamahendiran; Anna Chytil; Yun-Chen Chiang; Diana C Chong; Victoria L Bautch; W Kimryn Rathmell; Stephen Aylward; Ryan C Gessner; Paul A Dayton Journal: IEEE Trans Biomed Eng Date: 2018-07-27 Impact factor: 4.538