Literature DB >> 2825652

Fructose-2,6-bisphosphatase and 6-phosphofructo-2-kinase are separable in yeast.

M Kretschmer1, W Schellenberger, A Otto, R Kessler, E Hofmann.   

Abstract

Fructose-2,6-bisphosphatase was purified from yeast and separated from 6-phosphofructo-2-kinase and alkaline phosphatase. The enzyme released Pi from the 2-position of fructose 2,6-bisphosphate and formed fructose 6-phosphate in stoichiometric amounts. The enzyme displays hyperbolic kinetics towards fructose 2,6-bisphosphate, with a Km value of 0.3 microM. It is strongly inhibited by fructose 6-phosphate. The inhibition is counteracted by L-glycerol 3-phosphate. Phosphorylation of the enzyme by cyclic-AMP-dependent protein kinase causes inactivation, which is reversible by the action of protein phosphatase 2A.

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Year:  1987        PMID： 2825652      PMCID： PMC1148341          DOI： 10.1042/bj2460755

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Affinity chromatography of phosphofructokinase using Cibacron blue F3G-A.

Authors:  H J Böhme; G Kopperschläger; J Schulz; E Hofmann
Journal:  J Chromatogr       Date:  1972-06-28

3.  The protein phosphatases involved in cellular regulation. Antibody to protein phosphatase-2A as a probe of phosphatase structure and function.

Authors:  S Alemany; H Y Tung; S Shenolikar; S J Pilkis; P Cohen
Journal:  Eur J Biochem       Date:  1984-11-15

4.  Formation of fructose 2,6-bisphosphate from fructose 1,6-bisphosphate by intramolecular cyclisation followed by alkaline hydrolysis.

Authors:  E Van Schaftingen; H G Hers
Journal:  Eur J Biochem       Date:  1981-07

5.  Isolation and characterization of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine liver.

Authors:  P D Kountz; M R el-Maghrabi; S J Pilkis
Journal:  Arch Biochem Biophys       Date:  1985-05-01       Impact factor: 4.013

6.  Fructose-2,6-bisphosphatase from rat liver.

Authors:  E van Schaftingen; D R Davies; H G Hers
Journal:  Eur J Biochem       Date:  1982-05

7.  Activation of yeast 6-phosphofructo-2-kinase by protein kinase and phosphate.

Authors:  S Yamashoji; B Hess
Journal:  FEBS Lett       Date:  1984-12-10       Impact factor: 4.124

8.  The mechanism by which glucose increases fructose 2,6-bisphosphate concentration in Saccharomyces cerevisiae. A cyclic-AMP-dependent activation of phosphofructokinase 2.

Authors:  J François; E Van Schaftingen; H G Hers
Journal:  Eur J Biochem       Date:  1984-11-15

9.  Fructose 2-phosphate, an intermediate of the dephosphorylation of fructose 2,6-bisphosphate with a purified yeast enzyme.

Authors:  C Purwin; M Laux; H Holzer
Journal:  Eur J Biochem       Date:  1987-04-01

10.  Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate.

Authors:  S J Pilkis; M R El-Maghrabi; J Pilkis; T Claus
Journal:  J Biol Chem       Date:  1981-04-25       Impact factor: 5.157
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  2 in total

1.  On the mechanism by which a heat shock induces trehalose accumulation in Saccharomyces cerevisiae.

Authors:  M J Neves; J François
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857

2.  Cyclic AMP, fructose-2,6-bisphosphate and catabolite inactivation of enzymes in the hydrocarbon-assimilating yeast Candida maltosa.

Authors:  E Polnisch; K Hofmann
Journal:  Arch Microbiol       Date:  1989       Impact factor: 2.552
  2 in total

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