Literature DB >> 28255722

Measurement of Lytic Granule Convergence After Formation of an NK Cell Immunological Synapse.

Hsiang-Ting Hsu1,2, Alexandre F Carisey1,2, Jordan S Orange3,4,5.   

Abstract

Natural killer (NK) cells contain specialized lysosome-related organelles termed lytic granules allowing them to mediate cytotoxicity against tumorigenic or virally infected target cells. NK cells polarize their lytic granules toward a target cell via the microtubule-organizing center (MTOC). Prior to that, however, lytic granules converge to the MTOC along microtubules utilizing minus-end-directed microtubule motors. Herein we describe how to visualize and quantify lytic granule convergence using confocal microscopy to gain quantitative insight into NK cell cytotoxicity and its regulation.

Entities:  

Keywords:  Confocal microscopy; Convergence; Immunological synapse; Lytic granules; MTOC; Mathematical algorithm

Mesh：

Year:  2017        PMID： 28255722      PMCID： PMC5861262          DOI： 10.1007/978-1-4939-6881-7_31

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

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2.  NIH Image to ImageJ: 25 years of image analysis.

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3.  Distinct integrin-dependent signals define requirements for lytic granule convergence and polarization in natural killer cells.

Authors:  Hsiang-Ting Hsu; Jordan S Orange
Journal:  Sci Signal       Date:  2014-10-07       Impact factor: 8.192

4.  Rapid activation receptor- or IL-2-induced lytic granule convergence in human natural killer cells requires Src, but not downstream signaling.

Authors:  Ashley Mentlik James; Hsiang-Ting Hsu; Prachi Dongre; Gulbu Uzel; Emily M Mace; Pinaki P Banerjee; Jordan S Orange
Journal:  Blood       Date:  2013-02-04       Impact factor: 22.113

5.  HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing.

Authors:  Hyoungjun Ham; Walter Huynh; Renee A Schoon; Ronald D Vale; Daniel D Billadeau
Journal:  J Immunol       Date:  2015-03-11       Impact factor: 5.422

6.  NK cell lytic granules are highly motile at the immunological synapse and require F-actin for post-degranulation persistence.

Authors:  Emily M Mace; Winona W Wu; Tina Ho; Shaina S Mann; Hsiang-Ting Hsu; Jordan S Orange
Journal:  J Immunol       Date:  2012-10-12       Impact factor: 5.422

7.  Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:  Ashley N Mentlik; Keri B Sanborn; Erika L Holzbaur; Jordan S Orange
Journal:  Mol Biol Cell       Date:  2010-05-05       Impact factor: 4.138

8.  Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.

Authors:  Gregory D Rak; Emily M Mace; Pinaki P Banerjee; Tatyana Svitkina; Jordan S Orange
Journal:  PLoS Biol       Date:  2011-09-13       Impact factor: 8.029

9.  Actin depletion initiates events leading to granule secretion at the immunological synapse.

Authors:  Alex T Ritter; Yukako Asano; Jane C Stinchcombe; N M G Dieckmann; Bi-Chang Chen; C Gawden-Bone; Schuyler van Engelenburg; Wesley Legant; Liang Gao; Michael W Davidson; Eric Betzig; Jennifer Lippincott-Schwartz; Gillian M Griffiths
Journal:  Immunity       Date:  2015-05-19       Impact factor: 31.745

10.  Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.

Authors:  Amit Tuli; Jerome Thiery; Ashley M James; Xavier Michelet; Mahak Sharma; Salil Garg; Keri B Sanborn; Jordan S Orange; Judy Lieberman; Michael B Brenner
Journal:  Mol Biol Cell       Date:  2013-10-02       Impact factor: 4.138
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  4 in total

Review 1.  Quantitative Imaging Approaches to Study the CAR Immunological Synapse.

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Journal:  Mol Ther       Date:  2017-06-26       Impact factor: 11.454

Review 2.  Actin Cytoskeleton Straddling the Immunological Synapse between Cytotoxic Lymphocytes and Cancer Cells.

Authors:  Hannah Wurzer; Céline Hoffmann; Antoun Al Absi; Clément Thomas
Journal:  Cells       Date:  2019-05-16       Impact factor: 6.600

Review 3.  The Right Partner in Crime: Unlocking the Potential of the Anti-EGFR Antibody Cetuximab via Combination With Natural Killer Cell Chartering Immunotherapeutic Strategies.

Authors:  Hasan Baysal; Ines De Pauw; Hannah Zaryouh; Marc Peeters; Jan Baptist Vermorken; Filip Lardon; Jorrit De Waele; An Wouters
Journal:  Front Immunol       Date:  2021-09-07       Impact factor: 7.561

4.  CD56 regulates human NK cell cytotoxicity through Pyk2.

Authors:  Justin T Gunesch; Amera L Dixon; Tasneem Am Ebrahim; Melissa M Berrien-Elliott; Swetha Tatineni; Tejas Kumar; Everardo Hegewisch-Solloa; Todd A Fehniger; Emily M Mace
Journal:  Elife       Date:  2020-06-08       Impact factor: 8.140
  4 in total

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