Literature DB >> 2824292

A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis.

C Sasakawa1, M Yoshikawa.   

Abstract

A series of variants of transposon Tn5 were constructed by replacement of the 2.7-kb central segment which encodes kanamycin resistance with various other resistance-coding genes: tetracycline, chloramphenicol, gentamicin, trimethoprim, streptomycin or ampicillin. A thermosensitive replication mutant of the broad-host-range transmissible plasmid R388 was also constructed for use as a suicide vector for the delivery of transposable elements.

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Year:  1987        PMID： 2824292     DOI： 10.1016/0378-1119(87)90145-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  35 in total

1.  The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein.

Authors:  E W Crawford; L J Shimkets
Journal:  Genes Dev       Date:  2000-02-15       Impact factor: 11.361

2.  Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2).

Authors:  Y Arakawa; M Ohta; R Wacharotayankun; M Mori; N Kido; H Ito; T Komatsu; T Sugiyama; N Kato
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

3.  Genetic analysis of photosynthesis in Rhodospirillum centenum.

Authors:  F H Yildiz; H Gest; C E Bauer
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

4.  Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants.

Authors:  A K Koch; O Käppeli; A Fiechter; J Reiser
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

5.  Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1.

Authors:  J N Weiser; E C Gotschlich
Journal:  Infect Immun       Date:  1991-07       Impact factor: 3.441

6.  Low-temperature induction of Myxococcus xanthus developmental gene expression in wild-type and csgA suppressor cells.

Authors:  H G Rhie; L J Shimkets
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

7.  Role of ornibactin biosynthesis in the virulence of Burkholderia cepacia: characterization of pvdA, the gene encoding L-ornithine N(5)-oxygenase.

Authors:  P A Sokol; P Darling; D E Woods; E Mahenthiralingam; C Kooi
Journal:  Infect Immun       Date:  1999-09       Impact factor: 3.441

8.  H-NS regulation of virulence gene expression in enteroinvasive Escherichia coli harboring the virulence plasmid integrated into the host chromosome.

Authors:  B Colonna; M Casalino; P A Fradiani; C Zagaglia; S Naitza; L Leoni; G Prosseda; A Coppo; P Ghelardini; M Nicoletti
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

9.  Isolation and characterization of a new chromosomal virulence gene of Agrobacterium tumefaciens.

Authors:  I G Wirawan; H W Kang; M Kojima
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

10.  A novel approach to insertional mutagenesis of Haemophilus influenzae.

Authors:  C Sharetzsky; T D Edlind; J J LiPuma; T L Stull
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490
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