Literature DB >> 1847369

A novel approach to insertional mutagenesis of Haemophilus influenzae.

C Sharetzsky1, T D Edlind, J J LiPuma, T L Stull.   

Abstract

Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in the production of haemocin were detected. This strategy provides an efficient mechanism for the insertional mutagenesis of H. influenzae.

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Year:  1991        PMID： 1847369      PMCID： PMC207296          DOI： 10.1128/jb.173.4.1561-1564.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  33 in total

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Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

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Journal:  Mol Gen Genet       Date:  1979

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Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

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Journal:  Bacteriol Rev       Date:  1972-12

Review 5.  Bacteriophage mu as a transposition element.

Authors:  A I Bukhari
Journal:  Annu Rev Genet       Date:  1976       Impact factor: 16.830

6.  Construction of DNA recognition sites active in Haemophilus transformation.

Authors:  D B Danner; H O Smith; S A Narang
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

7.  Translocation specificity of the Tn3 element: characterization of sites of multiple insertions.

Authors:  C P Tu; S N Cohen
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Development of competence of Haemophilus influenzae.

Authors:  H T Spencer; R M Herriott
Journal:  J Bacteriol       Date:  1965-10       Impact factor: 3.490

10.  An eleven-base-pair sequence determines the specificity of DNA uptake in Haemophilus transformation.

Authors:  D B Danner; R A Deich; K L Sisco; H O Smith
Journal:  Gene       Date:  1980-11       Impact factor: 3.688
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  11 in total

1.  Genome scanning in Haemophilus influenzae for identification of essential genes.

Authors:  K A Reich; L Chovan; P Hessler
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

2.  Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis.

Authors:  Oleg V Kurnasov; Boris M Polanuyer; Shubha Ananta; Roman Sloutsky; Annie Tam; Svetlana Y Gerdes; Andrei L Osterman
Journal:  J Bacteriol       Date:  2002-12       Impact factor: 3.490

3.  Bacteriophage HP2 of Haemophilus influenzae.

Authors:  Bryan J Williams; Miriam Golomb; Thomas Phillips; Joshua Brownlee; Maynard V Olson; Arnold L Smith
Journal:  J Bacteriol       Date:  2002-12       Impact factor: 3.490

4.  Cloning and characterization of the haemocin immunity gene of Haemophilus influenzae.

Authors:  Y M Murley; T D Edlind; J M Pozsgay; J J LiPuma
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

5.  In vivo transposon mutagenesis in Haemophilus influenzae.

Authors:  A Kraiss; S Schlör; J Reidl
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

6.  The modA10 phasevarion of nontypeable Haemophilus influenzae R2866 regulates multiple virulence-associated traits.

Authors:  Timothy M VanWagoner; John M Atack; Kevin L Nelson; Hannah K Smith; Kate L Fox; Michael P Jennings; Terrence L Stull; Arnold L Smith
Journal:  Microb Pathog       Date:  2015-12-21       Impact factor: 3.738

7.  Effect of multiple mutations in the hemoglobin- and hemoglobin-haptoglobin-binding proteins, HgpA, HgpB, and HgpC, of Haemophilus influenzae type b.

Authors:  D J Morton; P W Whitby; H Jin; Z Ren; T L Stull
Journal:  Infect Immun       Date:  1999-06       Impact factor: 3.441

8.  Role of lgtC in resistance of nontypeable Haemophilus influenzae strain R2866 to human serum.

Authors:  Alice L Erwin; Simon Allen; Derek K Ho; Paul J Bonthuis; Paul J Bonthius; Justin Jarisch; Kevin L Nelson; David L Tsao; William C T Unrath; Michael E Watson; Bradford W Gibson; Michael A Apicella; Arnold L Smith
Journal:  Infect Immun       Date:  2006-09-11       Impact factor: 3.441

9.  Construction of Hermes shuttle vectors: a versatile system useful for genetic complementation of transformable and non-transformable Neisseria mutants.

Authors:  E M Kupsch; D Aubel; C P Gibbs; A F Kahrs; T Rudel; T F Meyer
Journal:  Mol Gen Genet       Date:  1996-03-20

10.  Expression of the Haemophilus influenzae transferrin receptor is repressible by hemin but not elemental iron alone.

Authors:  D J Morton; J M Musser; T L Stull
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441
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