Literature DB >> 2822040

Construction of a cDNA clone containing the entire coding region of the human liver-type phosphofructokinase.

D Levanon1, E Danciger, N Dafni, Y Groner.   

Abstract

Genomic fragments containing coding sequences of the human liver phosphofructokinase (PFKL) were used to screen cDNA libraries and several clones corresponding to PFKL were isolated. Three overlapping cDNA clones spanning the entire coding region were stitched together yielding the plasmid pG-cPFKL3.0 containing a 3Kb PFKL cDNA down stream of the T7 promoter. To assess its coding capacity pG-cPFKL3.0 was transcribed by T7 RNA polymerase and the RNA product was used to program an in vitro translation system. An 80KDa polypeptide which co-migrated with an in vivo labeled PFKL and was recognized by anti PFKL antibodies was synthesized. These results show that the cDNA clone pG-cPFKL3.0 contains the entire coding region of the human PFKL.

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Year:  1987        PMID： 2822040     DOI： 10.1016/s0006-291x(87)80194-8

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  A contiguous Not I restriction map of band q22.3 of human chromosome 21.

Authors:  D Wang; H Fang; C R Cantor; C L Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

2.  A DNA polymorphism with KpnI of the human liver-type phosphofructokinase (PFKL) gene.

Authors:  A C Warren; Y Groner; S E Antonarakis
Journal:  Nucleic Acids Res       Date:  1988-09-26       Impact factor: 16.971

3.  Molecular mapping of 21 features associated with partial monosomy 21: involvement of the APP-SOD1 region.

Authors:  Z Chettouh; M F Croquette; B Delobel; S Gilgenkrants; C Leonard; C Maunoury; M Prieur; M O Rethoré; P M Sinet; M Chery
Journal:  Am J Hum Genet       Date:  1995-07       Impact factor: 11.025

4.  Partial physical map of human chromosome 21 from fibroblast and lymphocyte DNA.

Authors:  N Crété; J M Delabar; Z Rahmani; M L Yaspo; J Kraus; A Marks; P M Sinet; N Créau-Goldberg
Journal:  Hum Genet       Date:  1993-04       Impact factor: 4.132

5.  Novel clustering of Sp1 transcription factor binding sites at the transcription initiation site of the human muscle phosphofructokinase P1 promoter.

Authors:  J L Johnson; A McLachlan
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

6.  No significant effect of monosomy for distal 21q22.3 on the Down syndrome phenotype in "mirror" duplications of chromosome 21.

Authors:  C Pangalos; D Théophile; P M Sinet; A Marks; D Stamboulieh-Abazis; Z Chettouh; M Prieur; C Verellen; M O Rethoré; J Lejeune
Journal:  Am J Hum Genet       Date:  1992-12       Impact factor: 11.025

7.  Analysis of human chromosome 21: correlation of physical and cytogenetic maps; gene and CpG island distributions.

Authors:  K Gardiner; M Horisberger; J Kraus; U Tantravahi; J Korenberg; V Rao; S Reddy; D Patterson
Journal:  EMBO J       Date:  1990-01       Impact factor: 11.598
  7 in total

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