| Literature DB >> 28218898 |
Jared T Simpson1,2, Rachael E Workman3, P C Zuzarte1, Matei David1, L J Dursi1, Winston Timp3.
Abstract
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.Entities:
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Year: 2017 PMID： 28218898 DOI： 10.1038/nmeth.4184
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547