| Literature DB >> 28214995 |
Yu-Chi Gao1,2, Yan Jia1,2, De-Qian Xiao1, Xin Wang1,2, You-Chao Dai1,2, Shi-Yan Yu1,2, Chen Chen1,2, Ze-Gang Zhuang1,2, Xiao-Xia Fu2, Jun-Ai Zhang1,2, Bi-Ying Zheng1,2, Zhi-Hong Chen2, Ji-Xin Zhong3, Zhang-Quan Chen1,2, Jun-Fa Xu4,5.
Abstract
IL-37 has been described as a natural inhibitor of immune responses. Monoclonal antibody (mAb) against human IL-37b with high affinity and specificity can serve as a molecular probe to detect IL-37 and study IL-37 functions, mechanisms and related signal pathways in inflammatory diseases. However, there are very few such mAbs against human IL-37 commercially available so far. In the current study, monoclonal antibodies against human IL-37b were developed by fusing splenocytes from immunized mouse with SP2/0 myeloma cells and polyethylene glycol. Then the antibodies were screened with prokaryotic expressed human IL-37b protein and eukaryotic expressed human IL-37b protein subsequently. Western blot and flow cytometry analysis revealed that selected mAb clons were able to recognize human IL-37 with high specificity. And more importantly, the IL-37b mAbs were fluorescently labeled and can be directly used in flow cytometry and immunohistochemistry. In conclusion, the current study developed new mAbs against human IL-37b, which are applicable in flow cytometry and immunohistochemistry.Entities:
Keywords: ELISA; Flow cytometry; Hybridoma; Limiting dilution
Year: 2017 PMID： 28214995 PMCID： PMC5366961 DOI： 10.1007/s10616-016-0052-5
Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058