Kunwu Yu1, Xiaohong Min2, Yingzhong Lin3, Ying Huang4, Song Huang5, Ling Liu3, Yudong Peng1, Kai Meng1, Dazhu Li1, Qingwei Ji6, Qiutang Zeng1. 1. Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 2. Department of Pathology, Puren Hospital, Wuhan University of Science and Technology, Wuhan, China. 3. Department of Cardiology, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. 4. Department of Ultrasound, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. 5. Department of Orthopedics, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 6. Department of Cardiology, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. Electronic address: jqw124@163.com.
Abstract
BACKGROUND: Our previous study indicates that IL-37 plays a critical role in both atherosclerosis and arterial calcification. However, whether IL-37 concentrations are significantly changed in patients with arterial calcification has not yet been investigated. METHODS: Anterior tibial arterial wall specimens were obtained from 8 patients with type 2 diabetes mellitus and 8 patients who experienced a traffic accident. IL-37 expression was measured by immunohistochemistry in the calcified and the normal samples. In addition, plasma IL-37 concentrations were measured in 75 patients with coronary artery calcification (CAC) and 50 patients without coronary artery calcification (NCAC). RESULTS: High concentrations of IL-37 were detected in calcified samples, whereas low concentrations of IL-37 were detected in the normal arteries. Macrophages and vascular smooth muscle cells were the main source of IL-37. Plasma IL-37 concentrations were significantly increased in CAC patients compared with NCAC patients. A correlation analysis showed that IL-37 was positively correlated with age, fasting glucose, alkaline phosphatase, IL-6, TNF-α, C-reactive protein and Agatston scores. Binary logistic regression analyses demonstrated that fasting glucose and IL-37 were independently associated with the presence of CAC. CONCLUSIONS: Increased IL-37 concentrations are associated with the onset of arterial calcification.
BACKGROUND: Our previous study indicates that IL-37 plays a critical role in both atherosclerosis and arterial calcification. However, whether IL-37 concentrations are significantly changed in patients with arterial calcification has not yet been investigated. METHODS: Anterior tibial arterial wall specimens were obtained from 8 patients with type 2 diabetes mellitus and 8 patients who experienced a traffic accident. IL-37 expression was measured by immunohistochemistry in the calcified and the normal samples. In addition, plasma IL-37 concentrations were measured in 75 patients with coronary artery calcification (CAC) and 50 patients without coronary artery calcification (NCAC). RESULTS: High concentrations of IL-37 were detected in calcified samples, whereas low concentrations of IL-37 were detected in the normal arteries. Macrophages and vascular smooth muscle cells were the main source of IL-37. Plasma IL-37 concentrations were significantly increased in CAC patients compared with NCAC patients. A correlation analysis showed that IL-37 was positively correlated with age, fasting glucose, alkaline phosphatase, IL-6, TNF-α, C-reactive protein and Agatston scores. Binary logistic regression analyses demonstrated that fasting glucose and IL-37 were independently associated with the presence of CAC. CONCLUSIONS: Increased IL-37 concentrations are associated with the onset of arterial calcification.
Authors: Vienna E Brunt; Akpevweoghene P Ikoba; Brian P Ziemba; Dov B Ballak; Alexander Hoischen; Charles A Dinarello; Marissa A Ehringer; Douglas R Seals Journal: Geroscience Date: 2022-05-27 Impact factor: 7.581