Mohamed-Amine Jabri1, Dalanda Wannes2, Najla Hajji2, Mohsen Sakly3, Lamjed Marzouki4, Hichem Sebai2. 1. Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Tunisia; Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia. Electronic address: jabri.amino@gmail.com. 2. Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Tunisia; Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia. 3. Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia. 4. Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Tunisia.
Abstract
INTRODUCTION: Mallaw (Malva sylvestris L.) is a medicinal plant who is traditionally being used as an antiulcer, laxative and anti-hemorrhoid, besides of its culinary use as a food in Tunisian cuisine. The present study was carried out to evaluate the protective effect of Malva sylvestris aqueous extract (MSAE) on constipation- induced by loperamide in male Wistar rats. METHODS: Rats were divided into six groups of six animals each: Control, Loperamide (LOP), LOP+ yohimbine (2mg/kg b.w, i.p.) and LOP+ various doses of MSAE (100, 200 and 400mg/kg b.w, p.o.). The laxative activity was determined based on the weight, frequency and water content of the feces matter. The effects of MSAE were also evaluated on normal gastric emptying and gastro-intestinal transit as well as loperamide-induced serum metabolic parameters changes and colon oxidative stress. RESULTS: The MSAE (100, 200 and 400mg/kg; b.w, p.o.), significantly reduced loperamide-induced constipation in a dose-dependent manner. Our extract improves also the gastrointestinal motility, mitigates colon lipid peroxidation and H2O2 colon overload, preserve normal antioxidant enzymes activities and non-enzymatic antioxidant levels, as well as provides stability of lipid profile, hepatic (AST and ALT) and renal (urea and creatinine) levels changed by loperamide intoxication. CONCLUSION: We suggests in the present work that MSAE had a potent effect against Loperamide- induced constipation through in part to an increase of gastrointestinal motility, astimulation of water intestinal secretion as well as its antioxidant properties.
INTRODUCTION: Mallaw (Malva sylvestris L.) is a medicinal plant who is traditionally being used as an antiulcer, laxative and anti-hemorrhoid, besides of its culinary use as a food in Tunisian cuisine. The present study was carried out to evaluate the protective effect of Malva sylvestris aqueous extract (MSAE) on constipation- induced by loperamide in male Wistar rats. METHODS:Rats were divided into six groups of six animals each: Control, Loperamide (LOP), LOP+ yohimbine (2mg/kg b.w, i.p.) and LOP+ various doses of MSAE (100, 200 and 400mg/kg b.w, p.o.). The laxative activity was determined based on the weight, frequency and water content of the feces matter. The effects of MSAE were also evaluated on normal gastric emptying and gastro-intestinal transit as well as loperamide-induced serum metabolic parameters changes and colon oxidative stress. RESULTS: The MSAE (100, 200 and 400mg/kg; b.w, p.o.), significantly reduced loperamide-induced constipation in a dose-dependent manner. Our extract improves also the gastrointestinal motility, mitigates colon lipid peroxidation and H2O2 colon overload, preserve normal antioxidant enzymes activities and non-enzymatic antioxidant levels, as well as provides stability of lipid profile, hepatic (AST and ALT) and renal (urea and creatinine) levels changed by loperamide intoxication. CONCLUSION: We suggests in the present work that MSAE had a potent effect against Loperamide- induced constipation through in part to an increase of gastrointestinal motility, astimulation of water intestinal secretion as well as its antioxidant properties.
Authors: Ji Eun Kim; Yun Ju Choi; Su Jin Lee; Jeong Eun Gong; Young Ju Lee; Ji Eun Sung; Young Suk Jung; Hee Seob Lee; Jin Tae Hong; Dae Youn Hwang Journal: PLoS One Date: 2021-02-24 Impact factor: 3.240