Nermien E Waly1, Abeer Refaiy2, Nora M Aborehab3. 1. Department of Physiology, Faculty of Medicine, Helwan University, Cairo, 11795, Egypt. Electronic address: nwaly@icloud.com. 2. Department of Pathology Faculty of Medicine, Assiut University, Assiut, 71515, Egypt. Electronic address: abeer_refaiy@yahoo.com. 3. Department of Biochemistry, Faculty of Pharmacy, MSA University, Giza, 11787, Egypt. Electronic address: naborehab@msa.eun.eg.
Abstract
OBJECTIVE: Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor β-1 (TGF β-1). METHODS: Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 or 100mg/kg/day, p.o. for 2 month) was administered orally. Serum levels of Il-10 and TGF-β1 were determined by ELISA. Histopathological changes in treated and control joints were examined using hematoxylin-eosin (H & E) staining. RESULTS: The mean serum level of IL-10 significantly decreased in the OA group compared to control group (P value<0.0001). On the other hand, mean serum level of IL-10 significantly increased in GlcN treated groups when compared to the OA group (P value<0.0001). Serum level of TGF β-1 was significantly elevated in OA group compared to control group (P value<0.0001). On the other hand, the mean serum level of TGF β-1 was significantly decreased in the GlcN treated groups when compared to the OA group (P value<0.0001). Histopathological evaluation of GlcN treated groups showed different grades of healing, according to Osteoarthritis Research Society International (OARSI) grading system. CONCLUSION: Our results showed that IL-10 and TGF-β1 possibly mediate GlcN chondroprotective effects in OA. Both serum biomarkers can be useful in the follow-up of articular cartilage damage in clinical settings.
OBJECTIVE:Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor β-1 (TGF β-1). METHODS: Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 or 100mg/kg/day, p.o. for 2 month) was administered orally. Serum levels of Il-10 and TGF-β1 were determined by ELISA. Histopathological changes in treated and control joints were examined using hematoxylin-eosin (H & E) staining. RESULTS: The mean serum level of IL-10 significantly decreased in the OA group compared to control group (P value<0.0001). On the other hand, mean serum level of IL-10 significantly increased in GlcN treated groups when compared to the OA group (P value<0.0001). Serum level of TGF β-1 was significantly elevated in OA group compared to control group (P value<0.0001). On the other hand, the mean serum level of TGF β-1 was significantly decreased in the GlcN treated groups when compared to the OA group (P value<0.0001). Histopathological evaluation of GlcN treated groups showed different grades of healing, according to Osteoarthritis Research Society International (OARSI) grading system. CONCLUSION: Our results showed that IL-10 and TGF-β1 possibly mediate GlcN chondroprotective effects in OA. Both serum biomarkers can be useful in the follow-up of articular cartilage damage in clinical settings.
Authors: Tatiane Garcia Stancker; Stella Souza Vieira; Andrey Jorge Serra; Rafael do Nascimento Lima; Regiane Dos Santos Feliciano; José Antônio Silva; Solange Almeida Dos Santos; Marcia Ataize Dos Santos Vieira; Maíra Cecília Brandão Simões; Ernesto Cesar Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2018-03-08 Impact factor: 3.161