Victor Ricardo Manuel Muñoz Lora1, Juliana Trindade Clemente-Napimoga2, Henrique Ballassini Abdalla3, Cristina Gomes Macedo4, Giancarlo de la Torre Canales1, Celia Marisa Rizzatti Barbosa1. 1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, zip code 13414-903, Brazil. 2. São Leopoldo Mandic Institute and Research Center, Rua Jose Rocha Junqueira, 13 - Swift, Campinas, São Paulo, zip code 13045-755, Brazil; Laboratory of Orofacial Pain, Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, zip code 13414-903, Brazil. Electronic address: juliana.napimoga@slmandic.edu.br. 3. Laboratory of Orofacial Pain, Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, zip code 13414-903, Brazil. 4. São Leopoldo Mandic Institute and Research Center, Rua Jose Rocha Junqueira, 13 - Swift, Campinas, São Paulo, zip code 13045-755, Brazil; Laboratory of Orofacial Pain, Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, zip code 13414-903, Brazil.
Abstract
OBJECTIVE: This study aimed to investigate the antinociceptive effects of Botulinum toxin type A (BoNT-A) on persistent inflammatory hypernociception induced by arthritis in the temporomandibular joint (TMJ) of rats. MATERIAL AND METHODS: Wistar rats were induced to persistent inflammatory hypernociception in the left TMJ. Then, animals were treated with intra-TMJ injections of BoNT-A, using doses of 3.5, 7 and 14 U/kg. Saline was used as control group. Behavioral tests were applied to evaluated the effect of BoNT-A in the inflammatory hypernociception. After that, animals were euthanized and samples from peri-articular tissues and trigeminal ganglia were obtained for further analyses. RESULTS: BoNT-A reduced the persistent inflammatory hypernociception induced by arthritis in the TMJ of rats. BoNT-A significantly reduced the peripheral release of the neurotransmitters Substance P and Calcitonin gene related peptide; and the pro-inflammatory cytokine IL-1β. Otherwise, BoNT-A had no effect in the peripheral release of glutamate and the cytokine TNF-α. CONCLUSION: These results demonstrate that intra-articular injection of BoNT-A reduces the albumin-induced arthritis persistent hypernociception in TMJ of rats by peripheral inhibition of neuropeptides release.
OBJECTIVE: This study aimed to investigate the antinociceptive effects of Botulinum toxin type A (BoNT-A) on persistent inflammatory hypernociception induced by arthritis in the temporomandibular joint (TMJ) of rats. MATERIAL AND METHODS:Wistar rats were induced to persistent inflammatory hypernociception in the left TMJ. Then, animals were treated with intra-TMJ injections of BoNT-A, using doses of 3.5, 7 and 14 U/kg. Saline was used as control group. Behavioral tests were applied to evaluated the effect of BoNT-A in the inflammatory hypernociception. After that, animals were euthanized and samples from peri-articular tissues and trigeminal ganglia were obtained for further analyses. RESULTS: BoNT-A reduced the persistent inflammatory hypernociception induced by arthritis in the TMJ of rats. BoNT-A significantly reduced the peripheral release of the neurotransmitters Substance P and Calcitonin gene related peptide; and the pro-inflammatory cytokine IL-1β. Otherwise, BoNT-A had no effect in the peripheral release of glutamate and the cytokine TNF-α. CONCLUSION: These results demonstrate that intra-articular injection of BoNT-A reduces the albumin-induced arthritis persistent hypernociception in TMJ of rats by peripheral inhibition of neuropeptides release.
Authors: Luane Macêdo de Sousa; Joana Maria Dos Santos Alves; Conceição da Silva Martins; Karuza Maria Alves Pereira; Paula Goes; Delane Viana Gondim Journal: Inflamm Res Date: 2019-08-01 Impact factor: 4.575