Luane Macêdo de Sousa1, Joana Maria Dos Santos Alves2, Conceição da Silva Martins1, Karuza Maria Alves Pereira1,2, Paula Goes1,2, Delane Viana Gondim3,4. 1. Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil. 2. Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil. 3. Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil. delanegondim@yahoo.com.br. 4. Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil. delanegondim@yahoo.com.br.
Abstract
OBJECTIVE: To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ). MATERIALS AND METHODS: Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1. RESULTS: The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections. CONCLUSION: Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.
OBJECTIVE: To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovineserum albumin (mBSA) in rat temporomandibular joint (TMJ). MATERIALS AND METHODS:Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1. RESULTS: The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections. CONCLUSION: Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.
Authors: Marcela Gatica-Andrades; Dimitrios Vagenas; Jessica Kling; Tam T K Nguyen; Helen Benham; Ranjeny Thomas; Heinrich Körner; Bala Venkatesh; Jeremy Cohen; Antje Blumenthal Journal: Blood Adv Date: 2017-07-10
Authors: A Fassio; G Adami; D Gatti; G Orsolini; A Giollo; L Idolazzi; C Benini; E Vantaggiato; M Rossini; O Viapiana Journal: Int Immunopharmacol Date: 2018-12-31 Impact factor: 4.932
Authors: Marina C Oliveira; Luciana P Tavares; Juliana P Vago; Nathália V Batista; Celso M Queiroz-Junior; Angelica T Vieira; Gustavo B Menezes; Lirlândia P Sousa; Fons A J van de Loo; Mauro M Teixeira; Flávio A Amaral; Adaliene V M Ferreira Journal: PLoS One Date: 2016-01-07 Impact factor: 3.240
Authors: Ana Carolina de Figueiredo Costa; Luane Macedo de Sousa; Joana Maria Dos Santos Alves; Paula Goes; Karuza Maria Alves Pereira; Ana Paula Negreiros Nunes Alves; Mariana Lima Vale; Delane Viana Gondim Journal: Inflammation Date: 2021-06-02 Impact factor: 4.092