Literature DB >> 28203403

Clinic application of tissue engineered bronchus for lung cancer treatment.

Qiang Tan1, Ruijun Liu1, Xiaoke Chen1, Jingxiang Wu2, Yinggen Pan3, Shun Lu1, Walter Weder4, Qingquan Luo1.   

Abstract

BACKGROUND: Delayed revascularization process and substitute infection remain to be key challenges in tissue engineered (TE) airway reconstruction. We propose an "in-vivo bioreactor" design, defined as an implanted TE substitutes perfused with an intra-scaffold medium flow created by an extracorporeal portable pump system for in situ organ regeneration. The perfusate keeps pre-seeded cells alive before revascularization. Meanwhile the antibiotic inside the perfusate controls topical infection.
METHODS: A stage IIIA squamous lung cancer patient received a 5-cm TE airway substitute, bridging left basal segment bronchus to carina, with the in-vivo bioreactor design to avoid left pneumonectomy. Continuous intra-scaffold Ringer's-gentamicin perfusion lasted for 1 month, together with orthotopic peripheral total nucleated cells (TNCs) injection twice a week.
RESULTS: The patient recovered uneventfully. Bronchoscopy follow-up confirmed complete revascularization and reepithelialization four months postoperatively. Perfusate waste test demonstrated various revascularization growth factors secreted by TNCs. The patient received two cycles of chemotherapy and 30 Gy radiotherapy thereafter without complications related to the TE substitute.
CONCLUSIONS: In-vivo bioreactor design combines the traditionally separated in vitro 3D cell-scaffold culture system and the in vivo regenerative processes associated with TE substitutes, while treating the recipients as bioreactors for their own TE prostheses. This design can be applied clinically. We also proved for the first time that TE airway substitute is able to tolerate chemo-radiotherapy and suitable to be used in cancer treatment.

Entities:  

Keywords:  Trachea replacement; in-vivo bioreactor; lung cancer treatment; tissue engineering

Year:  2017        PMID: 28203403      PMCID: PMC5303100          DOI: 10.21037/jtd.2017.01.50

Source DB:  PubMed          Journal:  J Thorac Dis        ISSN: 2072-1439            Impact factor:   2.895


  21 in total

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2.  The fate of homograft tracheal transplants in sheep.

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Journal:  World J Surg       Date:  2008-08       Impact factor: 3.352

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Authors:  Pierre Delaere; Dirk Van Raemdonck
Journal:  J Thorac Dis       Date:  2016-03       Impact factor: 2.895

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Review 5.  Surgical management of locally advanced lung cancer.

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Journal:  Gen Thorac Cardiovasc Surg       Date:  2014-05-29

6.  The first tissue-engineered airway transplantation: 5-year follow-up results.

Authors:  Alessandro Gonfiotti; Massimo O Jaus; Daniel Barale; Silvia Baiguera; Camilla Comin; Federico Lavorini; Giovanni Fontana; Oriol Sibila; Giovanni Rombolà; Philipp Jungebluth; Paolo Macchiarini
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Review 7.  Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings.

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Journal:  Stem Cells       Date:  2013-01       Impact factor: 6.277

8.  Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues.

Authors:  Jordan S Miller; Kelly R Stevens; Michael T Yang; Brendon M Baker; Duc-Huy T Nguyen; Daniel M Cohen; Esteban Toro; Alice A Chen; Peter A Galie; Xiang Yu; Ritika Chaturvedi; Sangeeta N Bhatia; Christopher S Chen
Journal:  Nat Mater       Date:  2012-07-01       Impact factor: 43.841

Review 9.  Angiogenesis, cell differentiation and cell survival in tissue engineering and cancer research.

Authors:  Daniel Johannes Tilkorn
Journal:  GMS Interdiscip Plast Reconstr Surg DGPW       Date:  2015-08-24

10.  Clinical transplantation of a tissue-engineered airway.

Authors:  Paolo Macchiarini; Philipp Jungebluth; Tetsuhiko Go; M Adelaide Asnaghi; Louisa E Rees; Tristan A Cogan; Amanda Dodson; Jaume Martorell; Silvia Bellini; Pier Paolo Parnigotto; Sally C Dickinson; Anthony P Hollander; Sara Mantero; Maria Teresa Conconi; Martin A Birchall
Journal:  Lancet       Date:  2008-11-18       Impact factor: 79.321

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  5 in total

1.  Feasibility of Bioengineered Tracheal and Bronchial Reconstruction Using Stented Aortic Matrices.

Authors:  Emmanuel Martinod; Kader Chouahnia; Dana M Radu; Pascal Joudiou; Yurdagul Uzunhan; Morad Bensidhoum; Ana M Santos Portela; Patrice Guiraudet; Marine Peretti; Marie-Dominique Destable; Audrey Solis; Sabiha Benachi; Anne Fialaire-Legendre; Hélène Rouard; Thierry Collon; Jacques Piquet; Sylvie Leroy; Nicolas Vénissac; Joseph Santini; Christophe Tresallet; Hervé Dutau; Georges Sebbane; Yves Cohen; Sadek Beloucif; Alexandre C d'Audiffret; Hervé Petite; Dominique Valeyre; Alain Carpentier; Eric Vicaut
Journal:  JAMA       Date:  2018-06-05       Impact factor: 56.272

2.  Bioengineered carina reconstruction using In-Vivo Bioreactor technique in human: proof of concept study.

Authors:  Yuanyuan Xu; Zhiyi Guo; Ruijun Liu; Hongwu Wang; Sheng Wang; Walter Weder; Yingen Pan; Jingxiang Wu; Heng Zhao; Qingquan Luo; Qiang Tan
Journal:  Transl Lung Cancer Res       Date:  2020-06

Review 3.  The Growing Medical Need for Tracheal Replacement: Reconstructive Strategies Should Overcome Their Limits.

Authors:  Davide Adamo; Giulia Galaverni; Vincenzo Giuseppe Genna; Filippo Lococo; Graziella Pellegrini
Journal:  Front Bioeng Biotechnol       Date:  2022-05-09

4.  Transplantation of acellularized dermis matrix (ADM) plus fully covered metal stent to prevent stricture after circumferential endoscopic submucosal dissection of early esophageal cancer (with video).

Authors:  Xian-Bin Zhou; Shao-Wei Li; Sai-Qin He; Shan-Jing Xu; Yue Cai; Shi-Wen Xu; Xiao-Kang Li; Bin-Bin Gu; Xin-Li Mao; Li-Ping Ye
Journal:  Regen Ther       Date:  2021-10-23       Impact factor: 3.419

Review 5.  Current Strategies for Tracheal Replacement: A Review.

Authors:  Giuseppe Damiano; Vincenzo Davide Palumbo; Salvatore Fazzotta; Francesco Curione; Giulia Lo Monte; Valerio Maria Bartolo Brucato; Attilio Ignazio Lo Monte
Journal:  Life (Basel)       Date:  2021-06-25
  5 in total

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