Evaluation of the Effect of Radiofrequency Radiation Emitted From Wi-Fi Router and Mobile Phone Simulator on the Antibacterial Susceptibility of Pathogenic Bacteria Listeria monocytogenes and Escherichia coli.

Mobile phones and Wi-Fi radiofrequency radiation are among the main sources of the exposure of the general population to radiofrequency electromagnetic fields (RF-EMF). Previous studies have shown that exposure of microorganisms to RF-EMFs can be associated with a wide spectrum of changes ranged from the modified bacterial growth to the alterations of the pattern of antibiotic resistance. Our laboratory at the nonionizing department of the Ionizing and Non-ionizing Radiation Protection Research Center has performed experiments on the health effects of exposure to animal models and humans to different sources of electromagnetic fields such as cellular phones, mobile base stations, mobile phone jammers, laptop computers, radars, dentistry cavitrons, magnetic resonance imaging, and Helmholtz coils. On the other hand, we have previously studied different aspects of the challenging issue of the ionizing or nonionizing radiation-induced alterations in the susceptibility of microorganisms to antibiotics. In this study, we assessed if the exposure to 900 MHz GSM mobile phone radiation and 2.4 GHz radiofrequency radiation emitted from common Wi-Fi routers alters the susceptibility of microorganisms to different antibiotics. The pure cultures of Listeria monocytogenes and Escherichia coli were exposed to RF-EMFs generated either by a GSM 900 MHz mobile phone simulator and a common 2.4 GHz Wi-Fi router. It is also shown that exposure to RF-EMFs within a narrow level of irradiation (an exposure window) makes microorganisms resistant to antibiotics. This adaptive phenomenon and its potential threats to human health should be further investigated in future experiments. Altogether, the findings of this study showed that exposure to Wi-Fi and RF simulator radiation can significantly alter the inhibition zone diameters and growth rate for L monocytogenes and E coli. These findings may have implications for the management of serious infectious diseases.

Introduction

Antibiotic resistance is one of the most important threats to global health.[1] According to World Health Organization, this problem is rising dangerously to high levels worldwide, which leads to longer hospitalization, higher medical costs, and raised mortality.[2]

Bacteria are becoming resistant to almost all commonly available antibiotics and this is a worldwide problem.[1] Today, greater use of telecommunication technologies like Global System for Mobile communication (GSM), cordless phones, mobile base stations, wireless personal, and local area networks, such as bluetooth, has led to ever increasing exposure to radiofrequency electromagnetic fields (RF-EMF).[3] Therefore, living organisms are now being exposed to microwaves and radiofrequency radiation signals from various sources.[4] The effects of these radiations on the biological functions of living cells shows an emerging area of interest in human health with respect to environmental effects.[5] Several studies were conducted to confirm the effects of electromagnetic radiation on cell functions[6-8]; however, the findings obtained in these studies were controversial. In particular, it was proven that EMF can affect functional parameters (cell growth and antimicrobial susceptibility).[9-12]

Listeria monocytogenes is a gram-positive, facultative anaerobe, nonspore-forming, motile, and rod-shaped bacterium.[13] In 1952, it was recognized as the main cause of neonatal infection, meningitis, and sepsis.[14] Listeria infection in adult patients is related to immunocompromised systems like HIV infection,[15] organ transplants, individuals who have received corticosteroids, and immunosuppressant drugs for their malignancies. Escherichia coli known as E coli, a gram-negative, rod-shaped, facultatively anaerobic bacterium,[16] is a common cause of life-threatening infections such as bloodstream and urinary tract infections, otitis media, and other complications.[17]

Our laboratory at the nonionizing department of the Ionizing and Non-ionizing Radiation Protection Research Center has performed experiments on the health effects of exposure to animal models and humans to different sources of electromagnetic fields such as cellular phones,[18-20] mobile base stations,[21] mobile phone jammers,[22,23] laptop computers,[24] radars,[25] dentistry cavitrons,[26] magnetic resonance imaging,[27,28] and Helmholtz coils.[29,30] In this study, we assessed whether the exposure to 900 MHz and 2.4 GHz RF-EMF emitted from GSM and a common Wi-Fi router could change the susceptibility of microorganisms to different antibiotics.

Materials and Methods

Antibiotic Susceptibility Test

In the current study, L monocytogenes ATCC 19115 was used and E coli strain was isolated from patients in Faghihi hospital, Shiraz, Iran. Escherichia coli strain was characterized by conventional methods including morphological and biochemical tests and confirmed using API 20 E method. The pure cultures of L monocytogenes and E coli were diluted in Mueller-Hinton Broth to reach 0.5 McFarland turbidity standards to get 1.5 × 108 CFU/mL as the total count.[31] Bacterial suspensions were spread on plates and cultured with a set of 6 antimicrobial substances; they were tested by disk diffusion method (Kirby-Bauer method) on Mueller-Hinton agar (MHA-Biolife, Italy) plates and E coli ATCC 25922 was used as the quality control for antibiotic susceptibility tests, according to the Clinical and Laboratory Standards Institute guidelines (CLSI, 2013). The incubation period was 18 to 24 hours at 35°C, and then inhibition zones for each disk were measured.

Antimicrobial Agents

Antibiotics used for E coli tests were imipenem (10 μg), levofloxacin (LEVO 5 μg), aztreonam (30 μg), ciprofloxacin (CIPR 5 μg), cefotaxime (CTX 30 μg), and piperacillin (100 μg). Listeria monocytogenes tests were conducted using doxycycline (DOX 30 μg), sulfamethoxazole–trimethoprim (SXT 25 μg), LEVO 5μg, CTX 30 μg, CIPR 5 μg, and ceftriaxone (CTR 30 μg) antibiotics.

All antibiotic disks were purchased from ROSCO Diagnostica (DK-2630 Taastrup, Denmark). Results of antibiotic susceptibility tests before and after exposure to either Wi-Fi or GSM mobile phone radiation were measured and analyzed. The inhibition zone of each plate was recorded as the average of at least 2 different measurements (in millimeters). Three replicate agar plates were used for each regime, according to CLSI guidelines (2013).

Wi-Fi Router

A D-Link Wi-Fi router (D-Link, D-Link Corporation, Taiwan) was used in this study as the exposure source. During the exposure period, data were exchanged between the modem and a laptop computer that was placed in another room (5 m away from the Wi-Fi router).

The Wi-Fi router operated with a power level of 1 W and the specific absorption rate at the distance 14 cm between the bacterial suspension (broth medium) and Wi-Fi router was 0.13 W/kg. During the exposure, bacterial samples were collected in different times 3, 6, 9, and 12 hours after being exposed using sterile swabs.

Radiofrequency Simulator

In this study, all exposures were performed using a GSM 900 MHz mobile simulator operating in the “Talk mode.” This mobile phone simulator was developed at the Department of Medical Physics and Biomedical Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, by the collaboration of the private sector.

Outgrowth Curve

For the evaluation of radiofrequency exposure effect on the growth rate of bacteria, optical density (OD) was measured. For each bacterium, a precisely specified concentration of bacterial suspension inoculated in the broth medium and then divided into 2 series as a control and RF simulator exposure groups. For estimating the number of bacterial cells in a broth medium, the turbidity of each group was recorded in 625 nm absorption at different times using a spectrophotometer (UNICO UV-2100 Spectrophotometer, UNICO, USA).

Statistical Analysis

All experiments were replicated 3 times for exposed and nonexposed groups. The means were compared using the nonparametric Mann-Whitney U test, and statistical significance of any difference observed among the mean values was determined using SPSS version 15. P < .05 was considered significant.

Results and Discussions

In our study, we have evaluated E coli and L monocytogenes for their in vitro susceptibility to various antibiotics in the presence of radiofrequency radiation. For each antibiotic, inhibition zone was measured and the test was repeated 3 times. Data obtained for exposed and nonexposed (control) bacteria are summarized in Table 1.

Table 1.

Inhibition Zone Diameters Before and After Exposure to RF and Wi-Fi Radiofrequency Radiation for Escherichia coli.

Wi-Fi Exposure		RF Simulator			Wi-Fi Exposure
Exposure Time	Drug	Control (Mean ± SD)	Exposure (Mean ± SD)	P Value	Control (Mean ± SD)	Exposure (Mean ± SD)	P Value
3 hours	PIPRA	26.30 ± 0.58	24.67 ± 0.58	.0262a	25.67 ± 0.58	25.30 ± 0.58	.5608
	IMI	31.67 ± 0.58	25.30 ± 0.58	.0002a	29.67 ± 0.58	25.30 ± 0.58	.0008a
	LEVO	34.67 ± 0.58	30.30 ± 0.58	.0008a	34.67 ± 0.58	31.67 ± 0.58	.0032a
	AZT	35.30 ± 0.58	29.30 ± 0.58	.0002a	34.67 ± 0.58	32.30 ± 0.58	.0083a
	CIPR	33.67 ± 0.58	28.67 ± 0.58	.0005a	33.30 ± 1.20	30.67 ± 0.58	.0247a
	CTX	36.67 ± 0.58	31.30 ± 0.58	.0001a	34.67 ± 0.58	30.30 ± 0.58	.0008a
6 hours	PIPRA	26.30 ± 0.58	22.30 ± 0.58	.0011a	25.67 ± 0.58	24.67 ± 0.58	.1023
	IMI	31.67 ± 0.58	23.67 ± 0.58	.0001a	29.67 ± 0.58	26.67 ± 0.58	.0032a
	LEVO	34.67 ± 0.58	26.30 ± 0.58	.0001a	34.67 ± 0.58	30.67 ± 0.58	.0011a
	AZT	35.30 ± 0.58	25.67 ± 0.58	<.0001a	34.67 ± 0.58	30.67 ± 0.58	.0011a
	CIPR	33.67 ± 0.58	26.30 ± 0.58	.0001a	33.30 ± 1.20	33.67 ± 0.58	.7165
	CTX	36.67 ± 0.58	28.30 ± 0.58	.0001a	34.67 ± 0.58	29.30 ± 0.58	.0004a
9 hours	PIPRA	26.30 ± 0.58	22.67 ± 0.58	.0016a	25.67 ± 0.58	24.67 ± 0.58	.1023
	IMI	31.67 ± 0.58	25.67 ± 0.58	.0002a	29.67 ± 0.58	25.67 ± 0.58	.0011a
	LEVO	34.67 ± 0.58	28.30 ± 0.58	.0002a	34.67 ± 0.58	29.67 ± 0.58	.0005a
	AZT	35.30 ± 0.58	26.67 ± 0.58	.0001a	34.67 ± 0.58	28.67 ± 0.58	.0002a
	CIPR	33.67 ± 0.58	30.67 ± 0.58	.0032a	33.30 ± 1.20	30.30 ± 0.58	.0176a
	CTX	36.67 ± 0.58	28.67 ± 0.58	.0001a	34.67 ± 0.58	28.67 ± 0.58	.0002a
12 hours	PIPRA	26.30 ± 0.58	23.67 ± 0.58	.0051a	25.67 ± 0.58	24.30 ± 0.58	.0516
	IMI	31.67 ± 0.58	28.67 ± 0.58	.0032a	29.67 ± 0.58	25.67 ± 0.58	.0011a
	LEVO	34.67 ± 0.58	30.30 ± 0.58	.0008a	34.67 ± 0.58	32.30 ± 0.58	.0083a
	AZT	35.30 ± 0.58	27.67 ± 0.58	.0001a	34.67 ± 0.58	33.67 ± 0.58	.1023
	CIPR	33.67 ± 0.58	35.30 ± 0.58	.0262a	33.30 ± 1.20	34.30 ± 0.58	.2636
	CTX	36.67 ± 0.58	31.67 ± 0.58	.0005a	34.67 ± 0.58	35.30 ± 0.58	.2134

Abbreviations: AZT, aztreonam; CIPR, ciprofloxacin; CTX, cefotaxime; IMI, imipenem; LEVO, levofloxacin; PIPRA, piperacillin; RF, radiofrequency.

aStatistically significant diffidence.

According to Table 1, for E coli, exposure to Wi-Fi and RF simulator decreased the inhibition zone diameters that show an antibacterial resistance pattern. At first, there was no change in sensitivity, but after increasing the exposure time, a specific range of antibacterial resistance was observed.

After 24 hours of exposure, as it can be seen in Table 1 and Figures 1 and 2, the bacteria that were exposed to radiation showed less resistance compared to early-time exposure. However, they didn’t return to time 0 exposure condition.

Figure 1.

Inhibition zone diameters preexposure and postexposure to radiofrequency (RF) simulator radiation for Escherichia coli.

Figure 2.

Inhibition zone diameters preexposure and postexposure to Wi-Fi radiation for Escherichia coli.

According to Figures 3 and 4, for L monocytogenes, comparison of data obtained from exposed and nonexposed groups did not show any significant changes in their antibacterial activity except for DOX. However, for E coli, there was a significant change in antimicrobial activities that suggest exposure condition to radiation could influence the degree of antibiotic susceptibility of E coli more than Listeria. In a similar pattern, for L monocytogenes, a specific window of response was observed (Figures 3 and 4). Listeria monocytogenes response to each antibiotic was different, for DOX, and the window response occurred after 6 hours of exposure to Wi-Fi and RF simulator radiation. However, for other antibiotics, these changes were only observed at the ninth hour of exposure to Wi-Fi while this response could not be observed for RF simulator radiation. After 9 hours of exposure to Wi-Fi for CIPR and SXT antibiotics, bacteria had a tendency to become more resistant. This was in contrast to the pattern observed for LEVO, CTX, and CTR antibiotics, which an increased sensitivity was observed. As mentioned above, for Listeria, limited antibacterial changes were observed for DOX after exposure to Wi-Fi and RF simulator radiation. On the other hand, we have previously addressed the bioeffects of the exposure of bacteria to electromagnetic radiations and investigated different aspects of the challenging issue of the ionizing or nonionizing radiation-induced alterations in the susceptibility of microorganisms to antibiotics.[19,32-34]

Figure 3.

Inhibition zone diameters preexposure and postexposure to radiofrequency (RF) simulator radiation for Listeria monocytogenes.

Figure 4.

Inhibition zone diameters preexposure and postexposure to Wi-Fi radiation for Listeria monocytogenes.

In the current study, the pattern of the response of E coli to Wi-Fi and RF simulator radiation was identical. The maximum differences in the diameters of inhibition zones were observed between 6 and 9 hours of the bacterial exposure to radiation (Figures 1 and 2). After 12 hours of exposure, the bacterial responses to radiation as a stressor led to returning to the preexposure status. This observation is in line with the previous reports of Mortazavi et al,[18,19,34-36] who showed that the radiation-induced stimulatory/beneficial effects in bacteria can be observed only within a narrow window of radiation dose. Based on this theory, when the radiation level is within the window (between the lower and upper levels of the window), stimulatory effects of ionizing or nonionizing radiation can be detected. Therefore, the response of the bacteria and other microorganisms to any environmental stressors can be determined by some key factors such as the magnitude of the dose and dose rate. This type of response was previously confirmed in Klebsiella pneumoniae.[34]

We have also evaluated the effect of radiofrequency radiation on the growth rate of bacteria. As shown in Figures 5 and 6, during each investigated time period, remarkable differences were observed in the rate of bacterial growth in exposed and nonexposed groups (Table 2). In particular, gram-negative (E coli) and gram-positive bacteria (L monocytogenes) showed a significant growth after exposure. Moreover, the time to reach the logarithmic phase in the growth curve of the bacteria was faster in exposed groups. However, after 8 hours, based on OD625 absorbance, the total count of E coli bacteria in the exposed group was less than that of the control group. These observations are in line with the finding of Akbal et al.[37] However, the total counts of L monocytogenes after 24 hours of exposure was higher than that of the control group. At a broader view, our data confirm previous studies that showed that radiofrequency radiation could induce changes in cell growth and antibiotic sensitivity in E coli.

Figure 5.

Growth curves in Escherichia coli broth medium preexposure and postexposure.

Figure 6.

Growth curves Listeria monocytogenes in broth medium preexposure and postexposure.

Table 2.

Average Optical Density (OD625) Results for Escherichia coli and Listeria Monocytogenes Preexposure and Postexposure.

Experimental Results
Time	E Coli		L Monocytogenes
	OD625		OD625
	Control	Exposure	Control	Exposure
0 hour	0.003 ± 0.001	0.003 ± 0.006	0.002 ± 0.0011	0.002 ± 0.0006
1 hour	0.009 ± 0.006	0.004 ± 0.001	0.005 ± 0.006	0.008 ± 0.0006
2 hours	0.01 ± 0.001	0.016 ± 0.006	0.026 ± 0.001	0.042 ± 0.0003
3 hours	0.012 ± 0.006	0.035 ± 0.001	0.054 ± 0.001	0.075 ± 0.001
4 hours	0.049 ± 0.001	0.105 ± 0.006	0.081 ± 0.006	0.113 ± 0.001
5 hours	0.087 ± 0.001	0.201 ± 0.001	0.127 ± 0.001	0.14 ± 0.001
6 hours	0.194 ± 0.001	0.355 ± 0.002	0.194 ± 0.001	0.22 ± 0.002
7 hours	0.35 ± 0.01	0.38 ± 0.002	0.273 ± 0.001	0.3 ± 0.001
8 hours	0.41 ± 0.006	0.36 ± 0.002	0.235 ± 0.006	0.28 ± 0.001
9 hours	0.405 ± 0.006	0.36 ± 0.002	0.235 ± 0.001	0.27 ± 0.001
10 hours	0.4 ± 0.01	0.355 ± 0.002	0.236 ± 0.001	0.26 ± 0.002
11 hours	0.398 ± 0.003	0.35 ± 0.002	0.234 ± 0.006	0.24 ± 0.006
12 hours	0.39 ± 0.01	0.35 ± 0.002	0.235 ± 0.001	0.24 ± 0.001
24 hours	0.39 ± 0.01	0.345 ± 0.002	0.235 ± 0.001	0.217 ± 0.006

Some researchers have indicated that organisms acquire resistance through several known factors such as patient noncompliance or in vitro exposure to radiofrequency radiation.[38-40] Nowadays, our world is surrounded by enormous radiofrequency sources such as Wi-Fi routers and laptop computers that can lead to serious health problems. When someone is infected with a microorganism that obtained its resistance from the host environment, it causes a serious problem for health-care systems and treatment failure or receiving a higher dosage of antibiotics will be possible. Therefore, this may lead to more side effects and finally prolonged hospitalization.

In several studies,[10,41] it was shown that antimicrobial sensitivity alterations were affected by the intensity of electromagnetic fields. Antibacterial sensitivity also depends on the physical properties of the electromagnetic fields such as frequency and magnetic flux density, exposure duration, and type of bacteria. Based on this point, evaluation of the effect of radiofrequency radiation on bacteria is not only essential to investigate their environmental effects, but it is also vital for detecting the antibiotic resistance pattern in the clinical laboratories and environment.[42-45]

Since the frequency of Wi-Fi router is 2.4 GHz while it is 900 MHz for the mobile simulator, we can conclude that the difference in response to Wi-Fi and the mobile simulator is possibly due to the frequency of radiation.[46] In several studies on bacteria,[34,47,48] one of the factors that influenced antibacterial sensitivity was the cell wall structure of bacteria and peptidoglycan (PG) nature in gram-positive and gram-negative bacteria. In gram-positive ones like Listeria, cell wall thickness is greater than that of gram negatives. The thicker the PG,[49] the permeability of the cell wall to permit the entrance of molecules to the cells will be decreased. According to these findings, the frequency of radiation can make some changes in PG of cell wall and enhance the permeability of the membrane to antibiotics.[8,50] Torgomyan showed that alteration in the oxidoreduction state of proteins in the bacterial cell membrane can be the major membranous mechanism after exposure to low-intensity electromagnetic field.[51]

Also, the effect of electromagnetic radiation on E coli cultures was studied by Justo et al,[52] which found that cell growth could be changed (stimulation or inhibition) under magnetic field. Furthermore, the exposure of E coli ATCC 25992 to the magnetic field of 2 mT at the frequency of 50 Hz caused significant alterations in the morphology, growth curves, structural parameters, and the sensitivity to certain antibiotics such as nalidixic acid, amoxicillin, and erythromycin.[9,53] These results were confirmed by the study of Stansell et al,[54] who found that static magnetic fields at moderate intensities are able to decrease the antibiotic sensitivity and make E coli WHMC 4202 more resistant.

In our study, we used several antibiotics that act through various mechanisms including protein and DNA synthesis inhibition, cell wall inhibition, and dihydrofolate reductase inhibition (it is summarized in Table 3). Each antibiotic enters the cell via a specific pathway. Some of them enter via efflux pumps in the cell membrane,[34,55,56] and others enter via ion channels through the cell wall.[57] All of these antibiotics may enter the cell via a nonspecific mechanism such as endocytosis. In this mechanism, molecules pass the membrane based on the permeability of the cell wall.[58-60] Considering our results, we believe that Wi-Fi and mobile exposure can serve as physical methods to alter the antibacterial susceptibility of microorganisms. In this light, the permeability of the membrane can be changed by radiofrequency radiation. It seems that the radiation can alter the sensitivity of the efflux pumps or ion channels by permitting the entrance of the molecules through the cell wall. In order to verify these theories, it would be better if this study is replicated with other pathogenic bacteria both gram-positive and gram-negative ones with various forms of antibiotics.

Table 3.

Antibiotics Classification.

Antibiotic Classification	Mechanism	Antimicrobial Agents	Abbreviation
Sulfonamide	DHFRI	Trimethoprim/sulfamethoxazole	SXT
Penicillin	Inhibits cell wall synthesis	Ceftriaxone	CTR
		Cefotaxime	CTX
		Piperacillin	PIPRA
		Imipenem	IMI
		Aztreonam	AZT
Tetracycline	Protein synthesis inhibition (30 s)	Doxycycline	DOX
Fluoroquinolones	Nucleic acid synthesis inhibition	Ciprofloxacin	CIPR
		Levofloxacin	LEVO
Aminoglycoside	Protein synthesis inhibition (30 s)	Amikacin	AMI

Abbreviation: DHFRI, dihydrofolate reductase inhibitors.

Conclusion

Based on our results, it can be concluded that the bacterial strains used in this study respond differently to EMFs. These bacteria were capable of responding to environmental stresses that act by activating some specific systems such as ion channels, change via the membrane, DNA repair system, and probably ion efflux pumps in the membrane as well as interactions of molecules and antibacterial agents.[61] There are some ambiguities that need further investigations regarding answering questions such as which cellular mechanism is responsible for adaptation? Which factors are involved in alterations of antibacterial sensitivity? And subsequently, what are the differences in the response to radiation in gram-negative and gram-positive bacteria? Moreover, experiments on different bacterial strains with various electromagnetic fields should be performed in the future to better clarify these uncertainties.