Literature DB >> 28194429

Intracellular Assessment of ATP Levels in Caenorhabditis elegans.

Konstantinos Palikaras1, Nektarios Tavernarakis2.   

Abstract

Eukaryotic cells heavily depend on adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS) within mitochondria. ATP is the major energy currency molecule, which fuels cell to carry out numerous processes, including growth, differentiation, transportation and cell death among others (Khakh and Burnstock, 2009). Therefore, ATP levels can serve as a metabolic gauge for cellular homeostasis and survival (Artal-Sanz and Tavernarakis, 2009; Gomes et al., 2011; Palikaras et al., 2015). In this protocol, we describe a method for the determination of intracellular ATP levels using a bioluminescence approach in the nematode Caenorhabditis elegans.

Entities:  

Keywords:  ATP; Ageing; Caenorhabditis elegans; Energy homeostasis; Luciferase; Metabolism; Mitochondria

Year:  2016        PMID： 28194429      PMCID： PMC5303341          DOI： 10.21769/BioProtoc.2048

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors.

Authors:  C Lagido; J Pettitt; A J Porter; G I Paton; L A Glover
Journal:  FEBS Lett       Date:  2001-03-23       Impact factor: 4.124

2.  Method for measuring ATP production in isolated mitochondria: ATP production in brain and liver mitochondria of Fischer-344 rats with age and caloric restriction.

Authors:  Barry Drew; Christiaan Leeuwenburgh
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2003-07-10       Impact factor: 3.619

3.  The Energy Source for Bioluminescence in an Isolated System.

Authors:  W D McElroy
Journal:  Proc Natl Acad Sci U S A       Date:  1947-11       Impact factor: 11.205

Review 4.  Assay of mitochondrial ATP synthesis in animal cells and tissues.

Authors:  Cristofol Vives-Bauza; Lichuan Yang; Giovanni Manfredi
Journal:  Methods Cell Biol       Date:  2007       Impact factor: 1.441

5.  Coordination of mitophagy and mitochondrial biogenesis during ageing in C. elegans.

Authors:  Konstantinos Palikaras; Eirini Lionaki; Nektarios Tavernarakis
Journal:  Nature       Date:  2015-04-20       Impact factor: 49.962

6.  The double life of ATP.

Authors:  Baljit S Khakh; Geoffrey Burnstock
Journal:  Sci Am       Date:  2009-12       Impact factor: 2.142

7.  Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

Authors:  J R de Wet; K V Wood; D R Helinski; M DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

8.  Prohibitin couples diapause signalling to mitochondrial metabolism during ageing in C. elegans.

Authors:  Marta Artal-Sanz; Nektarios Tavernarakis
Journal:  Nature       Date:  2009-10-08       Impact factor: 49.962

9.  Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity.

Authors:  Haseeb Ahmad Khan
Journal:  J Biosci       Date:  2003-06       Impact factor: 1.826

10.  During autophagy mitochondria elongate, are spared from degradation and sustain cell viability.

Authors:  Ligia C Gomes; Giulietta Di Benedetto; Luca Scorrano
Journal:  Nat Cell Biol       Date:  2011-04-10       Impact factor: 28.824
  10 in total
  8 in total

1.  Mitochondrial Permeability Uncouples Elevated Autophagy and Lifespan Extension.

Authors:  Ben Zhou; Johannes Kreuzer; Caroline Kumsta; Lianfeng Wu; Kimberli J Kamer; Lucydalila Cedillo; Yuyao Zhang; Sainan Li; Michael C Kacergis; Christopher M Webster; Geza Fejes-Toth; Aniko Naray-Fejes-Toth; Sudeshna Das; Malene Hansen; Wilhelm Haas; Alexander A Soukas
Journal:  Cell       Date:  2019-03-28       Impact factor: 41.582

2.  A salt-induced kinase is required for the metabolic regulation of sleep.

Authors:  Jeremy J Grubbs; Lindsey E Lopes; Alexander M van der Linden; David M Raizen
Journal:  PLoS Biol       Date:  2020-04-21       Impact factor: 8.029

Review 3.  C. elegans as an Animal Model to Study the Intersection of DNA Repair, Aging and Neurodegeneration.

Authors:  Francisco José Naranjo-Galindo; Ruixue Ai; Evandro Fei Fang; Hilde Loge Nilsen; Tanima SenGupta
Journal:  Front Aging       Date:  2022-06-22

4.  Nanoluciferase-Based Method for Detecting Gene Expression in Caenorhabditis elegans.

Authors:  Ivana Sfarcic; Theresa Bui; Erin C Daniels; Emily R Troemel
Journal:  Genetics       Date:  2019-10-04       Impact factor: 4.562

5.  A C. elegans genome-wide RNAi screen for altered levamisole sensitivity identifies genes required for muscle function.

Authors:  Timothy Chaya; Shrey Patel; Erin M Smith; Andy Lam; Elaine N Miller; Michael Clupper; Kirsten Kervin; Jessica E Tanis
Journal:  G3 (Bethesda)       Date:  2021-04-15       Impact factor: 3.154

6.  Mild pentachlorophenol-mediated uncoupling of mitochondria depletes ATP but does not cause an oxidized redox state or dopaminergic neurodegeneration in Caenorhabditis elegans.

Authors:  Zachary R Markovich; Jessica H Hartman; Ian T Ryde; Kathleen A Hershberger; Abigail S Joyce; Patrick L Ferguson; Joel N Meyer
Journal:  Curr Res Toxicol       Date:  2022-08-02

7.  Physiological Dose of EGCG Attenuates the Health Defects of High Dose by Regulating MEMO-1 in Caenorhabditis elegans.

Authors:  Yan Lu; Yi Wang; Li-Gui Xiong; Jian-An Huang; Zhong-Hua Liu; Yu-Shun Gong
Journal:  Oxid Med Cell Longev       Date:  2021-06-24       Impact factor: 6.543

8.  Hypodermal responses to protein synthesis inhibition induce systemic developmental arrest and AMPK-dependent survival in Caenorhabditis elegans.

Authors:  Hans M Dalton; Sean P Curran
Journal:  PLoS Genet       Date:  2018-07-18       Impact factor: 5.917
  8 in total

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