| Literature DB >> 12799486 |
Abstract
Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based on in vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.Entities:
Mesh:
Substances:
Year: 2003 PMID: 12799486 DOI: 10.1007/bf02705114
Source DB: PubMed Journal: J Biosci ISSN: 0250-5991 Impact factor: 1.826