| Literature DB >> 28192422 |
Nai Yang Fu1,2, Anne C Rios1,2, Bhupinder Pal1,2, Charity W Law2,3, Paul Jamieson1, Ruijie Liu3, François Vaillant1,2, Felicity Jackling1, Kevin He Liu1, Gordon K Smyth4,5, Geoffrey J Lindeman1,6,7, Matthew E Ritchie3,5, Jane E Visvader1,2.
Abstract
Despite accumulating evidence for a mammary differentiation hierarchy, the basal compartment comprising stem cells remains poorly characterized. Through gene expression profiling of Lgr5+ basal epithelial cells, we identify a new marker, Tetraspanin8 (Tspan8). Fractionation based on Tspan8 and Lgr5 expression uncovered three distinct mammary stem cell (MaSC) subsets in the adult mammary gland. These exist in a largely quiescent state but differ in their reconstituting ability, spatial localization, and their molecular and epigenetic signatures. Interestingly, the deeply quiescent MaSC subset (Lgr5+Tspan8hi) resides within the proximal region throughout life, and has a transcriptome strikingly similar to that of claudin-low tumours. Lgr5+Tspan8hi cells appear to originate from the embryonic mammary primordia before switching to a quiescent state postnatally but can be activated by ovarian hormones. Our findings reveal an unexpected degree of complexity within the adult MaSC compartment and identify a dormant subset poised for activation in response to physiological stimuli.Entities:
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Year: 2017 PMID： 28192422 DOI： 10.1038/ncb3471
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824