Talita Castro1, Maria Cristina Domingues Fink2, Marilia Figueiredo3, Paulo Henrique Braz-Silva4, Cláudio Mendes Pannuti5, Karem Lopez Ortega6, Marina Gallottini7. 1. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: talita.correa@usp.br. 2. University of São Paulo, Laboratory of Virology, Institute of Tropical Medicine of São Paulo, Dr. Eneas Carvalho de Aguiar Avenue, 470, 05403-000, São Paulo-SP, Brazil. Electronic address: crisfink@usp.br. 3. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: marilia.figueiredo@usp.br. 4. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: pbraz@usp.br. 5. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: pannuti@usp.br. 6. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: klortega@usp.br. 7. University of São Paulo, School of Dentistry, Stomatology Department, Professor Lineu Prestes Avenue, 2227, 05508-000, São Paulo-SP, Brazil. Electronic address: mhcgmaga@usp.br.
Abstract
BACKGROUND: New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases. OBJECTIVE: The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test. STUDY DESIGN: Forty six subjects were included and distributed into 3 groups: 14 with CKF (Group 1), 12 with KT (Group 2) and 20 healthy individuals (Group 3). In a total, 315 samples were collected and analyzed through RT-PCR, being 151 of gingival crevicular fluid, 46 of saliva, 46 of mouthwash, 43 of blood and 29 of urine. RESULTS: All subjects from group 1 were positive for BKPyV in at least one collected samples and 14% were positive for JCPyV. In Group 2, 91.7% were positive for BKPyV and 51.7% for JCPyV. Among subjects of Group 3, 80% were positive for BKPyV and 45% for JCPyV. CONCLUSIONS: Oral fluids exhibited high prevalence of BKPyV and JCPyV and were equally efficient compared to urine and blood. The use of oral fluids to detect these polyomaviruses enhances positivity in screening, even in cases of absence of viremia and especially in individuals who are not able to urinate.
BACKGROUND: New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases. OBJECTIVE: The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test. STUDY DESIGN: Forty six subjects were included and distributed into 3 groups: 14 with CKF (Group 1), 12 with KT (Group 2) and 20 healthy individuals (Group 3). In a total, 315 samples were collected and analyzed through RT-PCR, being 151 of gingival crevicular fluid, 46 of saliva, 46 of mouthwash, 43 of blood and 29 of urine. RESULTS: All subjects from group 1 were positive for BKPyV in at least one collected samples and 14% were positive for JCPyV. In Group 2, 91.7% were positive for BKPyV and 51.7% for JCPyV. Among subjects of Group 3, 80% were positive for BKPyV and 45% for JCPyV. CONCLUSIONS: Oral fluids exhibited high prevalence of BKPyV and JCPyV and were equally efficient compared to urine and blood. The use of oral fluids to detect these polyomaviruses enhances positivity in screening, even in cases of absence of viremia and especially in individuals who are not able to urinate.
Authors: Giselle P A Pena; Gabriella S Mendes; Helver G Dias; Lucas S Gavazzoni; Ariane R Amorim; Norma Santos Journal: Eur J Clin Microbiol Infect Dis Date: 2018-10-18 Impact factor: 3.267
Authors: Ana Carolina Mamana; Maria Stincarelli; Dmitry José De Santana Sarmento; Alexandre Mendes Batista; Tânia Regina Tozetto-Mendoza; Marina Gallottini; José Osmar Medina De Abreu Pestana; Paulo Henrique Braz-Silva; Simone Giannecchini Journal: J Oral Microbiol Date: 2021-03-12 Impact factor: 5.474