Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.
Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.
Authors: Camila Freze Baez; Maria Angélica A M Guimarães; Rosângela A G Martins; Ana Carolina Jonard Zalona; João José Cossatis; Mariano Gustavo Zalis; Silvia Maria Baeta Cavalcanti; Rafael Brandão Varella Journal: J Med Virol Date: 2013-07-12 Impact factor: 2.327
Authors: Hans H Hirsch; Daniel C Brennan; Cinthia B Drachenberg; Fabrizio Ginevri; Jennifer Gordon; Ajit P Limaye; Michael J Mihatsch; Volker Nickeleit; Emilio Ramos; Parmjeet Randhawa; Ron Shapiro; Juerg Steiger; Manikkam Suthanthiran; Jennifer Trofe Journal: Transplantation Date: 2005-05-27 Impact factor: 4.939
Authors: Tatiana F Robaina; Gabriella S Mendes; Fabrício J Benati; Giselle A Pena; Raquel C Silva; Miguel A R Montes; Maria Elisa R Janini; Fernando P Câmara; Norma Santos Journal: J Med Virol Date: 2013-01 Impact factor: 2.327
Authors: Carla Prezioso; Marco Ciotti; Gabriele Brazzini; Francesca Piacentini; Sara Passerini; Alfonso Grimaldi; Doriana Landi; Carolina Gabri Nicoletti; Maria Antonella Zingaropoli; Marco Iannetta; Marta Altieri; Antonella Conte; Dolores Limongi; Girolama Alessandra Marfia; Maria Rosa Ciardi; Claudio Maria Mastroianni; Anna Teresa Palamara; Ugo Moens; Valeria Pietropaolo Journal: J Clin Med Date: 2022-01-11 Impact factor: 4.241