| Literature DB >> 28183591 |
Huijie Zhang1, Ting Yan1, Sha Xu1, Shini Feng1, Dandi Huang1, Morihisa Fujita1, Xiao-Dong Gao2.
Abstract
CpG oligodeoxynucleotides (ODNs) activate innate and adaptive immune responses, and show strong potential as immunotherapeutic agents against various diseases. Benefiting from their unique physicochemical properties, graphene oxide (GO) has recently attracted great attention in nanomedicine. In this study, we developed a novel CpG ODNs delivery system based on GO-chitosan (GO-CS) nanocomposites. GO-CS nanocomposites were prepared by self-assembly of both components via electrostatic interactions. Compared with GO, GO-CS nanocomposites possessed smaller size, positive surface charge and lower cytotoxicity. CpG ODNs were loaded onto GO-CS nanocomposites via electrostatic interactions. GO-CS nanocomposites greatly improved the loading capacity and cellular uptake of CpG ODNs. GO-CS/CpG ODNs complexes further resulted in an enhanced interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production compared with that of free CpG ODNs and GO/CpG ODNs complexes. Therefore, GO-CS nanocomposites can serve as efficient nanocarriers for enhancing the delivery efficiency of CpG ODNs.Entities:
Keywords: Chitosan; CpG oligodeoxynucleotides; Cytokines; Drug delivery; Graphene oxide
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Year: 2016 PMID： 28183591 DOI： 10.1016/j.msec.2016.12.072
Source DB: PubMed Journal: Mater Sci Eng C Mater Biol Appl ISSN： 0928-4931 Impact factor: 7.328