Literature DB >> 2817396

Inhibition of the streptavidin-biotin interaction by milk.

W L Hoffman1, A A Jump.   

Abstract

Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.

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Year:  1989        PMID： 2817396     DOI： 10.1016/0003-2697(89)90250-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Development of Improved Double-Nanobody Sandwich ELISAs for Human Soluble Epoxide Hydrolase Detection in Peripheral Blood Mononuclear Cells of Diabetic Patients and the Prefrontal Cortex of Multiple Sclerosis Patients.

Authors:  Dongyang Li; Christophe Morisseau; Cindy B McReynolds; Thomas Duflot; Jérémy Bellien; Rashed M Nagra; Ameer Y Taha; Bruce D Hammock
Journal:  Anal Chem       Date:  2020-04-27       Impact factor: 6.986

Review 2.  Emerging protein array technologies for proteomics.

Authors:  Jung-Rok Lee; Dewey Mitchell Magee; Richard Samuel Gaster; Joshua LaBaer; Shan X Wang
Journal:  Expert Rev Proteomics       Date:  2013-02       Impact factor: 3.940

3.  An adsorption chromatography assay to probe bulk particle transport through hydrogels.

Authors:  I Vladescu; O Lieleg; S Jang; Katharina Ribbeck
Journal:  J Pharm Sci       Date:  2011-09-08       Impact factor: 3.534

4.  A Bead-Based Flow Cytometric Assay for Monitoring Yersinia pestis Exposure in Wildlife.

Authors:  Jeffrey C Chandler; Laurie A Baeten; Doreen L Griffin; Thomas Gidlewski; Thomas J DeLiberto; Jeannine M Petersen; Ryan Pappert; John W Young; Sarah N Bevins
Journal:  J Clin Microbiol       Date:  2018-06-25       Impact factor: 5.948

5.  Antibody validation for Western blot: By the user, for the user.

Authors:  Lakshmi Pillai-Kastoori; Sam Heaton; Steve D Shiflett; Annabelle C Roberts; Alejandra Solache; Amy R Schutz-Geschwender
Journal:  J Biol Chem       Date:  2019-12-09       Impact factor: 5.157

6.  Phosphorylated tyrosine-containing proteins in primary lung cancer correlates with proliferation and prognosis.

Authors:  Y Gong; T Hirano; Y Kato; K Yoshida; Y Shou; T Ohira; N Ikeda; Y Ebihara; H Kato
Journal:  Br J Cancer       Date:  2002-06-17       Impact factor: 7.640
  6 in total

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