H F Miranda1,2, F Sierralta3,4, V Jorquera5, P Poblete5, J C Prieto5,6, V Noriega5,6. 1. Faculty of Medicine, School of Pharmacy, Andres Bello University, República 590, Santiago, Chile. hmiranda@med.uchile.cl. 2. Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Independencia 1027, Santiago, Chile. hmiranda@med.uchile.cl. 3. Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Independencia 1027, Santiago, Chile. 4. Faculty of Odontology, Finis Terrae University, Pedro de Valdivia 1509, Providencia, Chile. 5. Faculty of Medicine, School of Pharmacy, Andres Bello University, República 590, Santiago, Chile. 6. Cardiovascular Department, Clinic Hospital, University of Chile, Santos Dumont 999, Santiago, Chile.
Abstract
OBJECTIVE: Diabetic neuropathy (DN) is the most common complication of diabetes and pain is one of the main symptoms of diabetic neuropathy, however, currently available drugs are often ineffective and complicated by adverse events. The purpose of this research was to evaluate the antinociceptive interaction between gabapentin and minocycline in a mice experimental model of DN by streptozocin (STZ). METHODS: The interaction of gabapentin with minocycline was evaluated by the writhing and hot plate tests at 3 and 7 days after STZ injection or vehicle in male CF1 mice. RESULTS: STZ (150 mg/kg, i.p.) produced a marked increase in plasma glucose levels on day 7 (397.46 ± 29.65 mg/dL) than on day 3 (341.12 ± 35.50 mg/dL) and also developed neuropathic pain measured by algesiometric assays. Gabapentin produced similar antinociceptive activity in both writhing and hot plate tests in mice pretreated with STZ. However, minocycline was more potent in the writhing than in the hot plate test in the same type of mice. The combination of gabapentin with minocycline produced synergistic interaction in both test. CONCLUSION: The combination of gabapentin with minocycline in a 1:1 proportion fulfills all the criteria of multimodal analgesia and this finding suggests that the combination provide a therapeutic alternative that could be used for human neuropathic pain management.
OBJECTIVE:Diabetic neuropathy (DN) is the most common complication of diabetes and pain is one of the main symptoms of diabetic neuropathy, however, currently available drugs are often ineffective and complicated by adverse events. The purpose of this research was to evaluate the antinociceptive interaction between gabapentin and minocycline in a mice experimental model of DN by streptozocin (STZ). METHODS: The interaction of gabapentin with minocycline was evaluated by the writhing and hot plate tests at 3 and 7 days after STZ injection or vehicle in male CF1 mice. RESULTS:STZ (150 mg/kg, i.p.) produced a marked increase in plasma glucose levels on day 7 (397.46 ± 29.65 mg/dL) than on day 3 (341.12 ± 35.50 mg/dL) and also developed neuropathic pain measured by algesiometric assays. Gabapentin produced similar antinociceptive activity in both writhing and hot plate tests in mice pretreated with STZ. However, minocycline was more potent in the writhing than in the hot plate test in the same type of mice. The combination of gabapentin with minocycline produced synergistic interaction in both test. CONCLUSION: The combination of gabapentin with minocycline in a 1:1 proportion fulfills all the criteria of multimodal analgesia and this finding suggests that the combination provide a therapeutic alternative that could be used for humanneuropathic pain management.
Authors: Pascal Vanelderen; Tom Rouwette; Tamás Kozicz; René Heylen; Jan Van Zundert; Eric W Roubos; Kris Vissers Journal: Reg Anesth Pain Med Date: 2013 Mar-Apr Impact factor: 6.288
Authors: Stephanie A Eid; Phillipe D O'Brien; Lucy M Hinder; John M Hayes; Faye E Mendelson; Hongyu Zhang; Samanthi Narayanan; Steven F Abcouwer; Frank C Brosius; Subramaniam Pennathur; Masha G Savelieff; Eva L Feldman Journal: J Transl Sci Date: 2020-06-16