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Literature DB >> 28151466

Turning single-molecule localization microscopy into a quantitative bioanalytical tool.

Philip R Nicovich^1,2, Dylan M Owen³, Katharina Gaus^1,2.

Abstract

Single-molecule localization microscopy (SMLM) generates super-resolution images by serially detecting individual fluorescent molecules. The power of SMLM, however, goes beyond images: biologically relevant information can be extracted from the mathematical relationships between the positions of the fluorophores in space and time. Here we review the history of SMLM and how recent progress in methods for spatial point analysis has enabled quantitative measurement of SMLM data, providing insights into biomolecule patterning, clustering and oligomerization in biological systems.

Mesh：

Year: 2017 PMID： 28151466 DOI： 10.1038/nprot.2016.166

Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491

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Cited

46 in total

1. Quantifying Protein Copy Number in Super Resolution Using an Imaging-Invariant Calibration.

Authors: Francesca Cella Zanacchi; Carlo Manzo; Raffaella Magrassi; Nathan D Derr; Melike Lakadamyali
Journal: Biophys J Date: 2019-05-03 Impact factor: 4.033

2. A Theoretical High-Density Nanoscopy Study Leads to the Design of UNLOC, a Parameter-free Algorithm.

Authors: Sébastien Mailfert; Jérôme Touvier; Lamia Benyoussef; Roxane Fabre; Asma Rabaoui; Marie-Claire Blache; Yannick Hamon; Sophie Brustlein; Serge Monneret; Didier Marguet; Nicolas Bertaux
Journal: Biophys J Date: 2018-07-05 Impact factor: 4.033

3. Investigating Focal Adhesion Substructures by Localization Microscopy.

Authors: Hendrik Deschout; Ilia Platzman; Daniel Sage; Lely Feletti; Joachim P Spatz; Aleksandra Radenovic
Journal: Biophys J Date: 2017-12-05 Impact factor: 4.033

Review 4. Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors.

Authors: R Magrassi; S Scalisi; F Cella Zanacchi
Journal: Biophys Rev Date: 2019-09-16

5. Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction.

Authors: Janel L Davis; Yang Zhang; Sijia Yi; Fanfan Du; Ki-Hee Song; Evan A Scott; Cheng Sun; Hao F Zhang
Journal: Langmuir Date: 2020-02-25 Impact factor: 3.882

Turning single-molecule localization microscopy into a quantitative bioanalytical tool.

1. Quantifying Protein Copy Number in Super Resolution Using an Imaging-Invariant Calibration.

2. A Theoretical High-Density Nanoscopy Study Leads to the Design of UNLOC, a Parameter-free Algorithm.

3. Investigating Focal Adhesion Substructures by Localization Microscopy.

Review 4. Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors.

5. Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction.

Review 6. New approaches for solving old problems in neuronal protein trafficking.

7. Single Molecules Are Your Quanta: A Bottom-Up Approach toward Multidimensional Super-resolution Microscopy.

8. Super-resolution imaging of flat-mounted whole mouse cornea.

9. 3D active stabilization for single-molecule imaging.

Review 10. Super-resolution microscopy: a closer look at synaptic dysfunction in Alzheimer disease.