Literature DB >> 28139276

A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.

Erica Lourenço Fonseca1, Nilceia da Veiga Ramos2, Bruno G Nascimento Andrade3, Lena L C S Morais4, Michel F Abanto Marin5, Ana Carolina P Vicente6.   

Abstract

Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
Copyright © 2017 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Class A β-lactamase; Klebsiella species; Molecular identification; bla(LEN); bla(OKP); bla(SHV)

Mesh：

Substances：

Year:  2017        PMID： 28139276     DOI： 10.1016/j.diagmicrobio.2017.01.005

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  16 in total

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Journal:  Braz J Microbiol       Date:  2021-08-30       Impact factor: 2.214

2.  Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh.

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Journal:  Microbiol Resour Announc       Date:  2022-08-29

3.  Comparative Genomic Analysis of a Clinical Isolate of Klebsiella quasipneumoniae subsp. similipneumoniae, a KPC-2 and OKP-B-6 Beta-Lactamases Producer Harboring Two Drug-Resistance Plasmids from Southeast Brazil.

Authors:  Marisa F Nicolás; Pablo Ivan Pereira Ramos; Fabíola Marques de Carvalho; Dhian R A Camargo; Carlene de Fátima Morais Alves; Guilherme Loss de Morais; Luiz G P Almeida; Rangel C Souza; Luciane P Ciapina; Ana C P Vicente; Roney S Coimbra; Ana T Ribeiro de Vasconcelos
Journal:  Front Microbiol       Date:  2018-02-16       Impact factor: 5.640

4.  Whole-Genome Sequencing of Human Clinical Klebsiella pneumoniae Isolates Reveals Misidentification and Misunderstandings of Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae.

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Review 5.  Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Clinical Microbiology: What Are the Current Issues?

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Journal:  Ann Lab Med       Date:  2017-11       Impact factor: 3.464

6.  Molecular epidemiology of Klebsiella variicola obtained from different sources.

Authors:  Humberto Barrios-Camacho; Alejandro Aguilar-Vera; Marilu Beltran-Rojel; Edgar Aguilar-Vera; Josefina Duran-Bedolla; Nadia Rodriguez-Medina; Luis Lozano-Aguirre; Olga Maria Perez-Carrascal; Jesús Rojas; Ulises Garza-Ramos
Journal:  Sci Rep       Date:  2019-07-23       Impact factor: 4.379

7.  The ZKIR Assay, a Real-Time PCR Method for the Detection of Klebsiella pneumoniae and Closely Related Species in Environmental Samples.

Authors:  Elodie Barbier; Carla Rodrigues; Geraldine Depret; Virginie Passet; Laurent Gal; Pascal Piveteau; Sylvain Brisse
Journal:  Appl Environ Microbiol       Date:  2020-03-18       Impact factor: 4.792

8.  Complete Genome Sequences of Three Uropathogenic Klebsiella quasipneumoniae Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection.

Authors:  Sundharamani Venkitapathi; Belle M Sharon; Tahira A Ratna; Amanda P Arute; Philippe E Zimmern; Nicole J De Nisco
Journal:  Microbiol Resour Announc       Date:  2021-03-18

9.  Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (Enterobacter cloacae, Enterobacter hormaechei, Enterobacter roggenkampii, and Enterobacter kobei) Belonging to Enterobacter cloacae Complex With Clinical Significance.

Authors:  Yang Ji; Peihong Wang; Tingting Xu; Yanzi Zhou; Rongchang Chen; Huaiqiu Zhu; Kai Zhou
Journal:  Front Cell Infect Microbiol       Date:  2021-05-18       Impact factor: 5.293

10.  Complete Genomic Analysis of a Kingdom-Crossing Klebsiella variicola Isolate.

Authors:  Yatao Guo; Yao Zhai; Zhao Zhang; Daixi Li; Zhanwei Wang; Jingquan Li; Zilong He; Songnian Hu; Yu Kang; Zhancheng Gao
Journal:  Front Microbiol       Date:  2018-10-09       Impact factor: 5.640
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