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Literature DB >> 2813405

A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence.

Abstract

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.

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Year: 1989 PMID： 2813405 PMCID： PMC298304 DOI： 10.1073/pnas.86.21.8472

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

34 in total

1. Expression of small cytoplasmic transcripts of the rat identifier element in vivo and in cultured cells.

Authors: R D McKinnon; P Danielson; M A Brow; F E Bloom; J G Sutcliffe
Journal: Mol Cell Biol Date: 1987-06 Impact factor: 4.272

Review 2. Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information.

Authors: A M Weiner; P L Deininger; A Efstratiadis
Journal: Annu Rev Biochem Date: 1986 Impact factor: 23.643

A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence.

1. Expression of small cytoplasmic transcripts of the rat identifier element in vivo and in cultured cells.

Review 2. Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information.

Review 3. Nuclease hypersensitive sites in chromatin.

4. Molecular analysis of human repetitive sequence family and its use as genetic marker.

Review 5. Structure and function of repetitive DNA in eukaryotes.

6. A domain model for eukaryotic DNA organization: a molecular basis for cell differentiation and chromosome evolution.

7. Specificity of the cAMP-induced gene exposure reaction in CHO cells.

8. A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1.

9. Activity of two different silencer elements of the chicken lysozyme gene can be compensated by enhancer elements.

10. Lamin B constitutes an intermediate filament attachment site at the nuclear envelope.

1. The spatial distribution of exposed nuclear DNA in normal, cancer, and reverse-transformed cells.

2. A set of 4 nuclear proteins binds to a DNA sequence within the FOS promoter region.

3. Identification of a new, abundant superfamily of mammalian LTR-transposons.