Literature DB >> 1660958

A set of 4 nuclear proteins binds to a DNA sequence within the FOS promoter region.

J Radtke1, S Dooley, N Blin, G Unteregger.   

Abstract

We investigated DNA-protein-interactions occurring in the promoter region of c-fos using two-dimensional electrophoresis and south-western-blotting. When nuclear extracts from the human glioblastoma cell line HeRoSV were tested for their DNA-binding behaviour to a 650 bp-fragment within the promoter region of c-fos, we found 4 proteins designated as 120/6.6, 75/5.4, 65/6.4 55/5.0 interacting with this fragment. An additional protein 60/6.0 was detected by using a digoxygenine-labelled probe. These observations let us to assume that beside the well characterized SRF and FOS-JUN proteins additional factors recognize the promoter sequence and may play a role in c-fos regulation.

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Year:  1991        PMID： 1660958     DOI： 10.1007/bf00364844

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  20 in total

1.  A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.

Authors:  L M Staudt; H Singh; R Sen; T Wirth; P A Sharp; D Baltimore
Journal:  Nature       Date:  1986 Oct 16-22       Impact factor: 49.962

2.  The fos gene as "master switch".

Authors:  J L Marx
Journal:  Science       Date:  1987-08-21       Impact factor: 47.728

3.  A blotting procedure which preserves specific protein-DNA interactions.

Authors:  I Wendler; U Grossbach
Journal:  Anal Biochem       Date:  1988-07       Impact factor: 3.365

4.  Establishment and characterization of a human glioblastoma cell line with a stable karyotype and nullisomy 13.

Authors:  H Fischer; K Schwechheimer; M Heider; S Bernhardt; K D Zang
Journal:  Cancer Genet Cytogenet       Date:  1985-07

5.  Phosphorylation affects the DNA affinity of the nuclear protein 24/7 from human tumor cells.

Authors:  J Radtke; G Unteregger
Journal:  FEBS Lett       Date:  1988-08-29       Impact factor: 4.124

6.  Rapid detection of DNA-binding factors using protein-blotting and digoxigenin-dUTP marked probes.

Authors:  S Dooley; J Radtke; N Blin; G Unteregger
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

7.  Different types of modification in c-fos and its associated protein p39: modulation of DNA binding by phosphorylation.

Authors:  R Müller; R Bravo; D Müller; C Kurz; M Renz
Journal:  Oncogene Res       Date:  1987

8.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

9.  A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.

Authors:  M M Garner; A Revzin
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

10.  Identification and purification of a polypeptide that binds to the c-fos serum response element.

Authors:  R Treisman
Journal:  EMBO J       Date:  1987-09       Impact factor: 11.598
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