Muhammad Nasrum Massi1, Karya Triko Biatko2, Irda Handayani3, Muhammad Yogi Pratama1, Sari Septriani4, Gaby Maulida Nurdin4, Marina B Ali4. 1. Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia. 2. Department of Orthopedic and Traumotology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia. 3. Clinical Pathology Laboratory, Wahidin Sudirohusodo Hospital, Makassar 90245, Indonesia. 4. Medical Research Center, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
Abstract
PURPOSE: To detect Mycobacterium tuberculosis DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects. METHODS AND RESULTS: The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%. CONCLUSION: GeneXpert has a high sensitivity but low specificity value in this study.
PURPOSE: To detect Mycobacterium tuberculosis DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects. METHODS AND RESULTS: The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%. CONCLUSION: GeneXpert has a high sensitivity but low specificity value in this study.
Entities:
Keywords:
DNA M. tuberculosis; GeneXpert MTB/RIF; Specimen pus; Spondylitis TB
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