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Literature DB >> 21270230

Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system.

Doris Hillemann¹, Sabine Rüsch-Gerdes, Catharina Boehme, Elvira Richter.

Abstract

In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-based GeneXpert MTB/RIF (Xpert) assay system and conventional liquid and solid culture methods. Twenty (3.8%) of the 521 specimens gave no interpretable result. Whereas the sensitivity of the Xpert assay with tissue specimens was 69.0% (20 out of 29 culture-positive cases detected), 100% sensitivity was found with the urine and stool specimens. The combined sensitivity and specificity of the Xpert assay were calculated to be 77.3% and 98.2%, respectively.

Entities: Disease Gene

Mesh：

Year: 2011 PMID： 21270230 PMCID： PMC3122824 DOI： 10.1128/JCM.02268-10

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

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8. Technology for automated, rapid, and quantitative PCR or reverse transcription-PCR clinical testing.

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9. Polymerase chain reaction of pleural biopsy is a rapid and sensitive method for the diagnosis of tuberculous pleural effusion.

Authors: Nadia A Hasaneen; Maysaa E Zaki; Hala M Shalaby; Ahmad S El-Morsi
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Authors: Véronique Hofman; Eric Selva; Luce Landraud; Dominique Sicard; Nicolas Vénissac; Laurent Castillo; Alain Kermarec; Jérôme Mouroux; Pierre Dellamonica; Paul Hofman
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144 in total

1. Effectiveness of an integrated real-time PCR method for detection of the Mycobacterium tuberculosis complex in smear-negative extrapulmonary samples in an area of low tuberculosis prevalence.

Authors: Raquel Moure; Rogelio Martín; Fernando Alcaide
Journal: J Clin Microbiol Date: 2011-12-07 Impact factor: 5.948

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Authors: Kara M Palamountain; Jeff Baker; Elliot P Cowan; Shaffiq Essajee; Laura T Mazzola; Mutsumi Metzler; Marco Schito; Wendy S Stevens; Gloria J Young; Gonzalo J Domingo
Journal: J Infect Dis Date: 2012-03-07 Impact factor: 5.226

4. Xpert MTB/RIF assay for diagnosis of pleural tuberculosis.

Authors: Sven O Friedrich; Florian von Groote-Bidlingmaier; Andreas H Diacon
Journal: J Clin Microbiol Date: 2011-10-12 Impact factor: 5.948

5. Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

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Review 6. Xpert® MTB/RIF assay: development, evaluation and implementation of a new rapid molecular diagnostic for tuberculosis and rifampicin resistance.

Authors: Stephen D Lawn; Mark P Nicol
Journal: Future Microbiol Date: 2011-09 Impact factor: 3.165

7. A multisite assessment of the quantitative capabilities of the Xpert MTB/RIF assay.

Authors: Robert Blakemore; Pamela Nabeta; Amy L Davidow; Viral Vadwai; Rasim Tahirli; Vanisha Munsamy; Mark Nicol; Martin Jones; David H Persing; Doris Hillemann; Sabine Ruesch-Gerdes; Felicity Leisegang; Carlos Zamudio; Camilla Rodrigues; Catharina C Boehme; Mark D Perkins; David Alland
Journal: Am J Respir Crit Care Med Date: 2011-11-01 Impact factor: 21.405