Catherine M C Volgenant1, Egija Zaura2, Bernd W Brandt2, Mark J Buijs2, Marisol Tellez3, Gayatri Malik3, Amid I Ismail4, Jacob M Ten Cate2, Monique H van der Veen2. 1. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit, Amsterdam, the Netherlands. Electronic address: c.volgenant@acta.nl. 2. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit, Amsterdam, the Netherlands. 3. Department of Pediatric Dentistry and Community Oral Health Sciences, Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA. 4. Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA.
Abstract
OBJECTIVES: The relation between the presence of red fluorescent plaque and the caries status in children was studied. In addition, the microbial composition of dental plaque from sites with red fluorescent plaque (RFP) and from sites with no red fluorescent plaque (NFP) was assessed. METHODS: Fluorescence photographs were taken from fifty children (6-14 years old) with overnight plaque. Full-mouth caries scores (ICDAS II) were obtained. The composition of a saliva sample and two plaque samples (RFP and NFP) was assessed using 16S rDNA sequencing. RESULTS: At the site level, no clinically relevant correlations were found between the presence of RFP and the caries status. At the subject level, a weak correlation was found between RFP and the caries status when non-cavitated lesions were included (rs=0.37, p=0.007). The microbial composition of RFP differed significantly from NFP. RFP had more anaerobes and more Gram-negative bacterial taxa. The most discriminative operational taxonomic units (OTUs) for RFP were Corynebacterium, Leptotrichia, Porphyromonas and Selenomonas, while the most discriminative OTUs for NFP were Neisseria, Actinomyces, Streptococcus and Rothia. CONCLUSIONS: There were no clinical relevant correlations in this cross-sectional study between the presence of RFP and (early) caries lesions. There were differences in the composition of these phenotypically different plaque samples: RFP contained more Gram-negative, anaerobic taxa and was more diverse than NFP. CLINICAL SIGNIFICANCE: The study outcomes provide more insight in the possibilities to use plaque fluorescence in oral health risk assessments.
OBJECTIVES: The relation between the presence of red fluorescent plaque and the caries status in children was studied. In addition, the microbial composition of dental plaque from sites with red fluorescent plaque (RFP) and from sites with no red fluorescent plaque (NFP) was assessed. METHODS: Fluorescence photographs were taken from fifty children (6-14 years old) with overnight plaque. Full-mouth caries scores (ICDAS II) were obtained. The composition of a saliva sample and two plaque samples (RFP and NFP) was assessed using 16S rDNA sequencing. RESULTS: At the site level, no clinically relevant correlations were found between the presence of RFP and the caries status. At the subject level, a weak correlation was found between RFP and the caries status when non-cavitated lesions were included (rs=0.37, p=0.007). The microbial composition of RFP differed significantly from NFP. RFP had more anaerobes and more Gram-negative bacterial taxa. The most discriminative operational taxonomic units (OTUs) for RFP were Corynebacterium, Leptotrichia, Porphyromonas and Selenomonas, while the most discriminative OTUs for NFP were Neisseria, Actinomyces, Streptococcus and Rothia. CONCLUSIONS: There were no clinical relevant correlations in this cross-sectional study between the presence of RFP and (early) caries lesions. There were differences in the composition of these phenotypically different plaque samples: RFP contained more Gram-negative, anaerobic taxa and was more diverse than NFP. CLINICAL SIGNIFICANCE: The study outcomes provide more insight in the possibilities to use plaque fluorescence in oral health risk assessments.
Authors: Catherine M C Volgenant; Suzette V van der Waal; Bernd W Brandt; Mark J Buijs; Monique H van der Veen; N A M Rosema; Bernd L Fiebich; Thorsten Rose; Tim Schmitter; Max Gajfulin; Wim Crielaard; Egija Zaura Journal: Front Oral Health Date: 2022-03-30
Authors: C Zemouri; C M C Volgenant; M J Buijs; W Crielaard; N A M Rosema; B W Brandt; A M G A Laheij; J J De Soet Journal: J Oral Microbiol Date: 2020-05-13 Impact factor: 5.474