Qi Huang1, Jocelyn M Rodgers1,2, Russell J Hemley3, Toshiko Ichiye1. 1. Department of Chemistry, Georgetown University, Washington, DC, 20057. 2. Geophysical Laboratory, Carnegie Institution for Science, Washington, DC, 20015. 3. Department of Civil and Environmental Engineering, The George Washington University, Washington, DC, 20052.
Abstract
A critical question about piezophilic (pressure-loving) microbes is how their constituent molecules maintain function under high pressure. Here, factors are examined that may lead to the increased activity under pressure in dihydrofolate reductase from the piezophilic Moritella profunda compared to the homologous enzyme from the mesophilic Escherichia coli. Molecular dynamics simulations are performed at various temperatures and pressures to examine how pressure affects the flexibility of the enzymes from these two microbes, since both stability and flexibility are necessary for enzyme activity. The results suggest that collective motions on the 10-ns timescale are responsible for the flexibility necessary for "corresponding states" activity at the growth conditions of the parent organism. In addition, the results suggest that while the lower stability of many enzymes from deep-sea microbes may be an adaptation for greater flexibility at low temperatures, high pressure may enhance their adaptation to low temperatures.
A critical question about piezophilic (pressure-loving) microbes is how their constituent molecules maintain function under high pressure. Here, factors are examined that may lead to the increased activity under pressure inn class="Gene">dihydrofolate reductase from the piezophilic Moritella profunda compared to the homologous enzyme from the mesophilic Escherichia coli. Molecular dynamics simulations are performed at various temperatures and pressures to examine how pressure affects the flexibility of the enzymes from these two microbes, since both stability and flexibility are necessary for enzyme activity. The results suggest that collective motions on the 10-ns timescale are responsible for the flexibility necessary for "corresponding states" activity at the growth conditions of the parent organism. In addition, the results suggest that while the lower stability of many enzymes from deep-sea microbes may be an adaptation for greater flexibility at low temperatures, high pressure may enhance their adaptation to low temperatures.
Authors: Nozomi Ando; Buz Barstow; Walter A Baase; Andrew Fields; Brian W Matthews; Sol M Gruner Journal: Biochemistry Date: 2008-09-25 Impact factor: 3.162
Authors: Ole Christian Hagestad; Lingwei Hou; Jeanette H Andersen; Espen H Hansen; Bjørn Altermark; Chun Li; Eric Kuhnert; Russell J Cox; Pedro W Crous; Joseph W Spatafora; Kathleen Lail; Mojgan Amirebrahimi; Anna Lipzen; Jasmyn Pangilinan; William Andreopoulos; Richard D Hayes; Vivian Ng; Igor V Grigoriev; Stephen A Jackson; Thomas D S Sutton; Alan D W Dobson; Teppo Rämä Journal: IMA Fungus Date: 2021-08-09 Impact factor: 3.515