| Literature DB >> 28100412 |
Mohammadreza Sadeghi1, Beatrix Kapusinszky2, Danielle M Yugo3, Tung Gia Phan2, Xutao Deng2, Isis Kanevsky4, Tanja Opriessnig5, Amelia R Woolums6, David J Hurley7, Xiang-Jin Meng3, Eric Delwart8.
Abstract
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.Entities:
Keywords: Bosavirus; Cell culture; Metagenomics; Parvovirus
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Year: 2017 PMID: 28100412 PMCID: PMC5654489 DOI: 10.1016/j.biologicals.2016.12.009
Source DB: PubMed Journal: Biologicals ISSN: 1045-1056 Impact factor: 1.856