| Literature DB >> 28082412 |
Yong Jin Choi1, Chao-Po Lin2, Davide Risso3, Sean Chen1, Thomas Aquinas Kim1, Meng How Tan4, Jin Billy Li4, Yalei Wu5, Caifu Chen6, Zhenyu Xuan7, Todd Macfarlan8, Weiqun Peng9, K C Kent Lloyd10, Sang Yong Kim11, Terence P Speed12,13,14, Lin He2.
Abstract
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) efficiently generate all embryonic cell lineages but rarely generate extraembryonic cell types. We found that microRNA miR-34a deficiency expands the developmental potential of mouse pluripotent stem cells, yielding both embryonic and extraembryonic lineages and strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features of totipotent two-cell blastomeres. miR-34a restricts the acquisition of expanded cell fate potential in pluripotent stem cells, and it represses MERVL expression through transcriptional regulation, at least in part by targeting the transcription factor Gata2. Our studies reveal a complex molecular network that defines and restricts pluripotent developmental potential in cultured ESCs and iPSCs.Entities:
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Year: 2017 PMID： 28082412 PMCID： PMC6138252 DOI： 10.1126/science.aag1927
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728