Chunqiu Zhang1, Tingbin Zhang2, Shubin Jin1, Xiangdong Xue1, Xiaolong Yang1, Ningqiang Gong1, Jinchao Zhang3, Paul C Wang4,5, Jian-Hua Tian2, Jinfeng Xing2, Xing-Jie Liang1. 1. CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. 2. School of Chemical Engineering and Technology, Tianjin University , No. 92, Weijin Road, Nankai District, Tianjin 300072, China. 3. College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University , Baoding, 071002, China. 4. Laboratory of Molecular Imaging, Department of Radiology, Howard University , Washington, DC 20060, United States. 5. College of Science and Engineering, Fu Jen Catholic University , Taipei 24205, Taiwan.
Abstract
High-efficiency gene transfer and suitably low cytotoxicity are the main goals of gene transfection systems based on nonviral vectors. In addition, it is desirable to track the gene transfer process in order to observe and explain the mechanism. Herein, inspired by viral structures that are optimized for gene delivery, we designed a small-molecule gene vector (TR4) with aggregation-induced emission properties by capping a peptide containing four arginine residues with tetraphenylethene (TPE) and a lipophilic tail. This novel vector can self-assemble with plasmid DNA to form nanofibers in solution with low cytotoxicity, high stability, and high transfection efficiency. pDNA@TR4 complexes were able to transfect a variety of different cell lines, including stem cells. The self-assembly process induces bright fluorescence from TPE, which makes the nanofibers visible by confocal laser scanning microscopy (CLSM). This allows us for the tracking of the gene delivery process.
High-efficiency gene transfer and suitably low cytotoxicity are the main goals of gene transfection systems based on nonviral vectors. In addition, it is desirable to track the gene transfer process in order to observe and explain the mechanism. Herein, inspired by viral structures that are optimized for gene delivery, we designed a small-molecule gene vector (Chemical">pan class="Gene">TR4) with aggregation-induced emission properties by capping a peptide containing four arginine residues with tetraphenylethene (TPE) and a lipophilic tail. This novel vector can self-assemble with plasmid DNA to form nanofibers in solution with low cytotoxicity, high stability, and high transfection efficiency. pDNA@TR4 complexes were able to transfect a variety of different cell lines, including stem cells. The self-assembly process induces bright fluorescence from TPE, which makes the nanofibers visible by confocal laser scanning microscopy (CLSM). This allows us for the tracking of the gene delivery process.
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