Fei Ji1, Xinhua Yang2, Yan He1, Hui Wang3, Aixingzi Aili4, Yan Ding5. 1. Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, Urumqi, 830054, China. 2. Department of Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 201204, China. 3. Shanghai Yang Si Hospital, Shanghai, 200126, China. 4. Department of Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 201204, China. 1819483078@qq.com. 5. Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, Urumqi, 830054, China. 35030627@qq.com.
Abstract
PURPOSE: Differential methylation of both HOXA10 and catechol-O-methyltransferase (COMT) has been reported in different endometrium disorders, and the two genes are linked through the estrogen pathway. The current study investigates the DNA methylation of HOXA10 and COMT in ectopic and eutopic endometrial tissues and its correlation with and the occurrence of endometriosis in women from Xinjiang province in China. METHODS: In the current study, 120 patients with endometriosis were recruited from our hospital between January 2011 and June 2014. The DNA methylation sites of HOXA10 and COMT were detected using a DNA methylation array. The methylation levels of specific sites were compared between ectopic and eutopic endometrial tissues via pyrosequencing. RESULTS: Five differentially expressed CpGs were localized in the promoter region of the COMT gene and expressed significantly higher in the ectopic endometrium than the eutopic endometrium (P < 0.001). Two out of the five differentially expressed CpGs in the HOXA10 gene located in the promoter region were both significantly lower (nearly half) in the ectopic endometrium than the eutopic endometrium (P < 0.001). CONCLUSIONS: To summarize, significant differential methylation of HOXA10 and COMT promoter regions was found between the ectopic and eutopic endometrial tissues. This is the first study investigating the methylation of HOXA10 and COMT genes and their linkage to endometriosis in Chinese patients.
PURPOSE: Differential methylation of both HOXA10 and catechol-O-methyltransferase (COMT) has been reported in different endometrium disorders, and the two genes are linked through the estrogen pathway. The current study investigates the DNA methylation of HOXA10 and COMT in ectopic and eutopic endometrial tissues and its correlation with and the occurrence of endometriosis in women from Xinjiang province in China. METHODS: In the current study, 120 patients with endometriosis were recruited from our hospital between January 2011 and June 2014. The DNA methylation sites of HOXA10 and COMT were detected using a DNA methylation array. The methylation levels of specific sites were compared between ectopic and eutopic endometrial tissues via pyrosequencing. RESULTS: Five differentially expressed CpGs were localized in the promoter region of the COMT gene and expressed significantly higher in the ectopic endometrium than the eutopic endometrium (P < 0.001). Two out of the five differentially expressed CpGs in the HOXA10 gene located in the promoter region were both significantly lower (nearly half) in the ectopic endometrium than the eutopic endometrium (P < 0.001). CONCLUSIONS: To summarize, significant differential methylation of HOXA10 and COMT promoter regions was found between the ectopic and eutopic endometrial tissues. This is the first study investigating the methylation of HOXA10 and COMT genes and their linkage to endometriosis in Chinese patients.
Entities:
Keywords:
Catechol-O-methyltransferase; DNA methylation; Endometriosis; HOXA10
Authors: J Julie Kim; H S Taylor; Z Lu; O Ladhani; J M Hastings; K S Jackson; Y Wu; S W Guo; A T Fazleabas Journal: Mol Hum Reprod Date: 2007-03-09 Impact factor: 4.025
Authors: Matthew T Dyson; Damian Roqueiro; Diana Monsivais; C Mutlu Ercan; Mary Ellen Pavone; David C Brooks; Toshiyuki Kakinuma; Masanori Ono; Nadereh Jafari; Yang Dai; Serdar E Bulun Journal: PLoS Genet Date: 2014-03-06 Impact factor: 5.917